GBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweig
Abstract
The principle of a heterogeneous competitive enzyme immunoassay was
applied to a flow injection system. This flow injection immunoassay
(FIIA) can be run automatically. Antibodies to the triazine herbicide
atrazin were immobilized on membranes. Antibody containing membranes
were mounted in a cross-flow reactor and changed after each assay. In
the assay atrazin competed with an atrazin-peroxidase conjugate for the
antibody binding sites. The product of the enzyme reaction was measured
fluorimetrically. One assay is completed within 15 minutes. Sensitivities
of the FIIA proved similar to the ELISA and depended mainly
on the properties of the antibodies and conjugation strategies. The
potential and limitations of the FIIA system will be discussed
