GBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweig
Abstract
A flow injection analysis (FIA) system for the on-line determination of glucose in animalcell cultures is
described. The hydrogen peroxide generated in the enzyme reaction is determined via a highly sensitive
chemiluminescent reaction with luminol. Based on the measurement of the maximum emitted light intensity,
the system was able to analyse glucose in a linear range of 10° to 5x 102 M glucose concentration, with
an r.s.d. of 3% at the 1 mM level (5 measurements). The enzyme reactor used was stable for more than
4 weeks in continous operation. The FIA developed wasable to analyse up to 20 samples per hour. The
system has been sucessfully applied to on-line monitoring of glucose concentration during an animal cell
culture, designed for the production of human antithrombin III factor. Results obtained with the FIA
system were compared with off-line results, obtained with a Yellow Springs Instrument Company Model27
(YSI)
