GBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweig
Abstract
Since Flow-Injection Analysis (FIA) was introduced by Ruzicka and Hansenin 1975, a
large numberofapplications using biological active reactors have been discribed. These
reactors are often designedas fixed bed reactors with bio-material held between two nets
or immobilized in tubes or on membrane surfaces. These designs allow the use of the
enzyme’s naturalcatalytic activities. When denaturation, fowling or inhibition occure,
however, thereislittle chance of reactivating the enzyme and the entire reactor must be
discarded.
Ourintent to design a fully automated biosensor for pesticide detection using enzyme
inhibition made it necessary to develop methods for exchanging immobilized enzyme
between two runs of analysis