Additional file 1 of Stabilization of KPNB1 by deubiquitinase USP7 promotes glioblastoma progression through the YBX1-NLGN3 axis

Abstract

Additional file 1: Supplementary Fig. S1. KPNB1 regulated GBM progression in vitro. A-E. U87MG and U251MG cells were infected with lentivirus vectors expressing shKPNB1#1 and shKPNB1#2. Cells were collected for Western blot analysis (A), RT-qPCR (B), CCK8 assay (C), colony formation assay (D), and Transwell invasion assay (E). Data presented as the mean ± SD of three independent experiments, ***P < 0.001, one-way ANOVA. F-J. U87MG and U251MG cells were infected with lentivirus vectors expressing KPNB1 plasmids. Cells were collected for Western blot analysis (F), RT-qPCR (G), CCK8 assay (H), colony formation assay (I), and Transwell invasion assay (J). K and L. U87MG and U251MG cells were infected with lentivirus vectors expressing shKPNB1. The control and knockdown KPNB1 groups were treated with TMZ(10 μM), respectively. CCK8 was used to detect cell proliferation (K), and flow cytometry was used to detect cell apoptosis (L). Data presented as the mean ± SD of three independent experiments; ***P < 0.001, one-way ANOVA