Introduction: A potential application of plant cell and tissue culture to plant improvement is likely to be the use of variation generated by passage through cell and tissue culture (somaclonal variation). With the advent of regeneration protocols for soybean (Christianson et al., 1983; Lazzeri et al., 1985; Ranch et al., 1985, 1986; Barwale et al., 1986; Wright et al., 1986), it becomes necessary to document the occurrence and frequency of variant phenotypes in progeny of regenerated plants. We report here preliminary findings on the identification of a chromosomal variant in plants regenerated from embryogenic tissue cultures of soybean.</p