L-glutaminase is an important enzyme owing to their applications in pharmaceutical and food industries. In the present study, 142 L-glutaminase-producing bacterial strains were isolated from brackish water samples collected from the Lake Mariout at the North of Egypt. Results revealed that the majority of these strains produced the enzyme intracellularly and only 18 strains produced extracellular enzyme. Based on 16S rRNA gene analysis, the strains were identified as Citrobacter amalonaticus strain GPBW1, Legionella feeleii strain GPBW2, Enterobacter cloacae strain GPBW3, Empedobacter brevis strain GPBW4, Burkholderia plantarii strain GPBW5, Lysobacter antibioticus strain GPBW6, Vibrio parahaemolyticus strain GPBW7, Serratia fonticola strain GPBW8, Yokenella regensburgei strain GPBW9, and Klebsiella oxytoca strain GPBW10. The study highlighted the promising activity of Klebsiella oxytoca strain GPBW10 for L-glutaminase production
