Supplemental tables and figures for the paper "Changes in Rumen Epithelial Morphology and Transcriptome, Rumen Metabolome, and Blood Biochemical Parameters in Lactating Dairy Cows with Subacute Rumen Acidosis Following Rumen Content Transplantation".

Abstract

Supplemental Table S1. Ingredients and nutritional composition of the conventional diet (CON) and the high-grain diet (HG)Figure S1 Principal component (PC) analysis of total genes in the rumen epithelial papilla between the donor-recipient (DR) and the self-recipient (SR) groups. PERMANOVA results with 9999 permutations are shown.Figure S2 Heatmap-like functional classification of the differentially expressed genes (DEGs) in rumen epithelial papilla between the donor-recipient (DR) and the self-recipient (SR) groups. Expression patterns of the DEGs corresponding to the (A) significantly enriched Gene Ontology biological process terms and (B) KEGG pathways at a P value of Figure S3 The orthogonal partial least squares discriminant analysis (OPLS-DA) of rumen metabolites between the donor-recipient (DR) and the self-recipient (SR) groups. The score scatter plot of the OPLS-DA model in the (A) positive and (B) negative ion modes. Permutation test plots of 200 iterations in the (C) positive and (D) negative ion modes. R2 and Q2 are fitness power and predictive power of the model, respectively.Figure S4 Metabolic pathway analysis of KEGG for differential metabolites between the donor-recipient (DR) and the self-recipient (SR) groups.</p