The group composition of alcohol extracts from balsamic poplar leaves Populus balsamifera L, growing in the Krasnoyarsk Krai, Krasnoyarsk (Russia) were examined in this work. Leaves selected in different phases of the growing season were used: June, July, August – fresh, September – fallen leaves. Poplar leaves contain up to 56% of extractive substances, alcohol-soluble substances account for up to 67% of the extract amount. Alcohol extracts were fractionated using solvents with increasing polarity: petroleum ether (boiling point 40 to 70 °C), diethyl ether, ethyl acetate, butanol. The results showed that the alcohol extracts of poplar leaves are dominated by substances extracted with ethyl acetate (up to 60%). The study of the component composition of volatile compounds of individual fractions of alcohol extracts was performed on an Agilent chromatography-mass spectrometer. The presence of various groups of compounds was established: hydrocarbons, alcohols, acids, ketones, etc., including biologically active substances such as megasterol acetate, ℽ-sitosterol, sitosterol, 3,7,11,15-tetramethyl-2-hexadecene-1-ol, diethyltoluamide, 4-methoxy-3-nitrobiphenyl, and other compounds that it can be used in different applications