Several technical issues that affect eye-tracking have arisen concomitantly with the steadily increasing sizes of personal displays recently. One such issue is the loss of the illumination reflection of the near-infrared light-emitting diodes used as reference points around the edge of the display. Another issue is that reference identification is required for practical usage. Therefore, this paper proposes gaze estimation with transparent markers for large display environments to solve these problems. The transparent markers can be distributed on the screen, and a unique ID is assigned to each marker using linear polarization angles. The reference is detected using a polarization camera through the reflection on the cornea. The results of experiments conducted using a 50-inch display indicate that the proposed method can estimate the point-of-gaze to within 2.1 degrees of error. We confirmed that on-screen markers in sizable displays could be effectively used as references instead of illumination sources