Isolation and identifi cation of chemical compound as anticancer from the leaves of Kopasanda (Chromolaena odorata L.) had been done. Extraction was conducted successfully with n-hexane, ethylacetate and ethanol by maceration, and by refl ux for water solvent. Fractionation of ethylacetate extract by column chromatography (SiO2). n-hexane-ethylacetate = 10:1 ~ 1:1; ethylacetate). n-hexaneethylacetate = 5:1) gave one pure isolated fraction fr. 5-3 with inhibition activity against leukemia cell line L1210 at concentration 1.515 ppm. Chemical structure determination was done based on spectral data interpretation of Infra Red, 1D NMR (1H, 13C) and 2D NMR (H-H COSY, HMQC and HMBC) and mass spectra (LC-MS). Pure isolated fraction fr. 5-3 was deducted as methyl ether naragenine.Isolasi dan identifi kasi senyawa kimia sebagai antikanker dari daun Kopasanda (Chromolaena odorata L.) telah dilakukan. Ekstraksi dilakukan secara berturut-turut dengan n-heksan, etilasetat, dan etanol secara maserasi kecuali dengan air dilakukan secara refl uks. Fraksinasi ekstrak etilasetat dengan kromatografi kolom (SiO2,: i). n-heksan-etilasetat = 10:1 ~ 1:1; etilasetat, ii). n-heksan-etilasetat = 5:1) memberikan satu isolat murni fr. 5-3 yang mempunyai aktivitas daya hambat terhadap sel leukemia L1210 sebesar 1,515 bpj. Penentuan struktur kimia dilakukan berdasarkan interpretasi data spektra infra merah, RMI 1D (1H, 13C) dan RMI 2D (H-H COSY, HMQC dan HMBC) dan spektra masa (LC-MS). Isolat fr. 5-3 diduga adalah metil eter naragenin
