DATA AVAILABILITY : Raw Data is available on request.Fungi are ubiquitous in the environment and part of the commensal microfora on the conjunctiva of equine eyes. North
Queensland, being tropical, presents an ideal environment for fungi growth. When the cornea is injured, fungi can invade
the corneal stroma, resulting in keratomycosis. The objectives of this study were to determine the fungal species specifc to
the eyes of horses in the Townsville region; to investigate the potential risk factors associated with the presence of fungi;
and to test their susceptibility to antifungals to create an empirical guide for treatment. The eyes of forty ophthalmologically
normal horses from James Cook University were sampled throughout the summer months of December 2017, January 2018,
and January and February 2020. Cultured fungi were identifed morphologically, and their identity confrmed by comparing
partial 18sRNA DNA sequences with the NCBI nucleotide database. Minimum inhibitory concentration testing of common
antifungal medications was performed. Sixty-one out of eighty conjunctival samples grew fungi, and 21 diferent fungi
genera were isolated. The most common genera were Aspergillus (18%, 26/141), Curvularia (14%,20/141), Rhodotorula
(12%,17/141) and Penicillium (12%,17/141). No signifcant association was found between age or environmental factors
and fungal culture status. Most fungi were highly susceptible to voriconazole and ketoconazole but resistant to fuconazole
and amphotericin B. This adds to the body of evidence on which species of fungi are present as normal ocular microfora of
horses living in tropical regions of Australia, and an avenue for treating them.Open Access funding enabled and organized by CAUL and its Member Institutions.https://link.springer.com/journal/11259Veterinary Tropical Disease
