Expression of Some Molybdoenzyme Genes under Salt Stress Conditions in Chickpea, Bean and Lentil Plants

Abstract

The objective of this work is to isolate mARC, XDH1, AAO3, NIA1, SO and ABA3/LOS5 genes in three legume species submitted to salt stress with molybdenum and nitrogen, the growth conditions (T1: control, T2: both molybdenum and nitrogen added, T3: sodium chloride were added, T4: molybdenum, nitrogen and sodium chloride were added), we found these gene sequences in Phaseolus vulgaris and Cicer arietinum in NCBI but not forLens culinaris, so we tried to isolate them using bean and chickpea primers by reverse transcriptase PCR. In chickpea, aldehyde oxidase and xanthine dehydrogenase genes expression is enhanced by molybdenum and nitrogen. Nitrate reductase gene expression is affected by salinity. Sulfite oxidase and xanthine dehydrogenase are activated under salt stress in bean, which suggests that SO and XDH protein have a role in bean adaptation to salt stress. mARC gene expression is stimulated by presence of molybdenum and nitrogen, mARC2 bean protein and mARC chickpea protein may have a role in salt stress adaptation mechanism