Cattle tick, Rhipicephalus (Boophilus) microplus, and tick borne diseases are a major limitation on European breeds production in subtropical regions

BARIONI JUNIOR, W.

BORGES, L.

BORGES, M.

DOMINGUES, R.

GASPAR, E. B.

GULIAS GOMES, C. C.

LAUMANN, R. A.

MORAES, M. C. B.

OLIVEIRA, M. W. M. de

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2° Simpósio Embrapa LabEx EUA de Sanidade Animal28-30 de Agosto de 2012Embrapa Estudos e Capacitação. Brasí/ia - DFProspecting for bioactive molecules and biological indicator of host resis ancecontrol cattle tick, Rhipicephalus (Boophilus) microplus - Gomes C.C.G.l·, o eM.C.B.2, Borges M.2, Borges L.3, Laumann R.2, Barioni W.4, Wandré M.2, Gaspar E. .,Domingues R.l1 - Embrapa South Animal Husbandry & Sheep2 - Embrapa Genetic Resourses & Biotechnology3 - University of Goiânia4 - Embrapa Cattle Southeast* poster presenter: claudia@cppsul.embrapa.brCattle tick, Rhipicephalus (Boophilus) microplus, and tick borne diseases are a majorlimitation on European breeds production in subtropical regions. The parasitism also limitsthe expansion of crossbreeding with British breeds and their composites in tropical areas toimprove precocity and beef quality of zebu populations. Variation in host response toparasitism within breed has a genetic basis and is a heritable characteristic which enablesselection to increase parasite resistance in cattle population. The on-going project, funded byBrazilian Agricultural Research Corporation (Embrapa), addresses questions to find chemicalmarkers for tick resistance and natural tick repellent substances on body odor of Angusbreed. Phenotype for tick resistance of sixty narve heifers was defined by artificial infestation(AI). The estimated mean number of ticks was 245.9±10.41/animal (ranging from 79.9 to502). Chemical substances from the skin of each heifer were collected before and after AI byaeration system contained porapak adsorbent and by rubbing pieces of cotton cloth in theback of the animais. Substances were extracted with hexane and samples are under currentanalysis by gas chromatography- mass spectrometry. Quantitative and qualitative analysiswill be correlated to parasite load to define chemical profile of resistant individuais.Preliminary results showed at least two substances in higher concentration in resistant host.The project also includes behavioral tests to define larvae ability to discriminate body odor ofresistanUsusceptible heifers and comparison of skin resident microbiota on these extremegroups, possibly involved in odor composition. Both bioassays are still under development.Key-words: chemical markers, repellent, AngusEmbrapa project number: 03.09.01.022.00.0020 Simpósio Embrapa LabEx EUA de Sanidade Animal28-30 de Agosto de 2012Embrapa Estudos e Capacitação, Brasf/ia - DFProspecting for bioactive molecules and biological indicator of hostresistance to control cattle tick, Rhipicephalus (Boophilus)microplusc C Gulias Gomes". M. c. B. Moraes', M. Borges2.L. Borges3. R. Laumann2•W. Barioni', M. Wandre'. E. B. Gaspar", R. Oomingues'1. Embrapa Soulh Animal Husbandry & ShHp. 2. Embrapa G.,1fIIic RoliOUfS8S & Bi<bchnoIogy; 3. Univetsityof Goiária; 4. Embrapa C.I/Ie SouIheasl• pos;er prestJl1lor: claudia.quI •• mbmpa brINTRODUCTIONThe parasl1lsmby cattle tIck, Rnip/cepn8lus (BoophHus) microplus Isa major limitation on Europaan breeds production. It also limits lheexpanslon of crossbreeding with British breeds and !heir composltes intropical areas to improve precocity and beel quality 01zebu populatlons.Varlatlon In host response to parasnlsm wilhln breed has a genetic basisand Is a herltable characteristlc whlch enables seiection to incresseparaslte reslstance In cattle population.The on-going project addresses questlons to fInd chemlcal marl<ersfor tlck reslstance and natural tick repelient substances on body odor 01Angus breed.METHODS AND STRATEGYOdoul'"u •••••cot~..,o,..AIOdour •••••coltetlon "' ••. AIt-too. ••••• , •••••• ~ ••••••••••••••;' ••••• .-fI.,~.,,~ .........•..'..•....... -.-..,..,• ••••1oOIII~;N14·,.0ID•...•-" ,.....,.. •......•·,.tntrUêt)"r'INIIldtc;;o..-~\~I IE_ophyoiology:slngIe c.l1 RKording·~I(I.JC~r-s-""·1_ ••Ifl...,.~."oIIIAIm:(1) to Investigate ta,.,.. .bility todiscriminate body odor ot resistant.'MlJoepbbfeNtlferS, (2) to IMI repetlentlattradive effect ofcM1Ie odor oomponenlSAm: (1) To Icl.-clfy chem.caI markers dtQõl AI~"I.iInc8, (2) Pr~ngrape 5ent ~stancn ftIrtic;1I:coovolPhenotypa lor tick resistance 01 sixty naíve heilelS was defined byartiftcial infestation (AI). Chemical substances lrom lhe skin 01 eachheilel _Ie collecled by air entrainment and by rubbing pieces 01cottondoth in lhe back 01lhe animais. Substances were extracled with hexaneand sampíes are under current analysis by gas chromatography-massspectrometry. Quantitative and qualitative analysis wili ba correlated toparasite load to define chemical prefile of resistanlmdividuals.'The projecl also Indudes behavioral leslS to define larvae abi~ty 10discriminata body odor 01resistantlsusceplible heifers and comparison ofskin resident microbiota on Ihese extreme groups, possibly involved inodor composltlon. Samples 01skin bacteria were coIIectedlrom neck andinner hind legs region using swabs 60th bioassays are stil underdevelopmenlt. Tri<IK>1omyond """deaning befor. odor00110<:",,"PRELIMINARY RESULTS56••! ~,/1 36! 31.,." 2'i!' ,.<l 11•,100 200 300 400Tâcounts.00Figurv 1. Mean number of ücks carried by the artificially infested Angul heifarsaResislant SusoeptibIeaI,. BAgure 2. VoIatUo c:ompounds cofleded by alr entrolnmcnt from Angusheiters (5 reslstant and 5 susoeptibfe). Dlfferent letters on barsIndicate slgnlficant d11ferences between groups (rosistant Xsusceptible. P<O.05, Mann-M'\i1ney test).Dlrectlsol._ BHI agar Itol.tionBIood _ Macconkey BIood _ "'aoconkcyFtgure 3. IUustration of IkIn mlcrobkJta tsOtation of Angus heders..

Prospecting for bioactive molecules and biological indicator of host resistance control cattle tick, Rhipicephalus (Boophilus) microplus.

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Prospecting for bioactive molecules and biological indicator of host resistance control cattle tick, Rhipicephalus (Boophilus) microplus.

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