FALA, A. M.

LANDIM-ALVARENGA, F. C.

MACHADO, R.

OLIVEIRA, C. A.

PASCHOAL, D. M.

SUDANO, M. J.

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Re-utilization of norgestomet implants in superovulated Nelore cowsM.J. Sudanol, D.M. Paschoar, A.M. Falal, C.A. Oliveira3, F.C. Landim-Alvarengal, R. MachadolDRARV, FMVZ-UNESP, Botucatu, SP, Brazil; 2Embrapa, S~o Carlos, SP, Brazil; 3VRA, FMVZ-USP, Sao Paulo, SP. BraziIntroduction:The re-utilization of progestin implants is an alternative to reduce costs in protocols to induce ovulation in cows.The objective of this study was to evaluate the re-utilization ofnotgestomet implants in Nelore cows superovulatedwith eCG. The methodology proposed aimed the reduction of costs with supe~:bvulation protocols in Nelore embryotransfer programs. [ilMaterials and Methods:The experiment was performed at Embrapa experimental farm, located in Sao Carlos in the southeast of Brazil.Eight Nelore cows (Bos tourus indicus) were randomly distributed in two experimental groups. Group 1: On DO, arandom day of the estrus cycle, half of the cows received a new norgestomet implant (Crestar@, 1ntervet) and 2 mg1M of estradiol benzoate (BE) (Estrogin@, Farmavet) and Group 2: On DO the other half received 2 norgestometimplants, which were used before during 8 days, and also 2 mg of BE. On D4 cows from both groups received onesingle dose of2000 ill ofeCG (FoUigon@, Intervet). On D6 two doses ofPGF2a (150 /.lg) (Sincrocio@, Ourofmo)were administrated. The implants were removed 36 hours after the first PGFza and 12.5 mg ofLH 1M (Lutropin-V@,Bioniche) was administered 48 hours after the PGFza. The cows were artificially inseminated 12 to 24 hours afterLH injection with semen from just one bull. The number of follicles with ability to ovulate was estimated throughtransrectal ultrasonography (MINDRA Y DP 3300) at the moment of LH administration (D8) and the ovulation ratewas estimated on D15. The cows were flushed on D15 and blood samples were collected for progesteroneradioimunoassay using LDH from FMVZ-USP. The results were analyzed through unpaired t Test and Fisher'sExact Test with a significant level of 5% (P < 0.05).Results and Discussion: .The superovulatory response was higher on Group1. The mean number of corpus luteum (CL) and ovulation ratewas also higher on Group 1. The results indicate that the superovulatory response with the re-utilization of 2norgestomet implants in Nelore Cows is less intense when compared with the use of just one new implant. -Group 1(1 new implant) Group 2 (2 used implants)N° follicles >Smm 17.1:f:l.Sa 9.7:f:l.6bFollicular diameter (mm) 10.5:f:1.6 10.2:f:l.7N°CL 13.S:f:1.7a 5.5:J::1.0bCLdiameter(mm)' 15.7:f:1.S i4.4:J:: 1.7Ovulation rate (%) SO.3a .53.SbN° recovered structures 6.3 :f: 1.1 4.0:f: 1.9N° transferable embryos 4.0:f: 1.S 2.5 :f: 1.1Pro esterone n mI. 36.1~S.3 35.1:f:6.9a. ifferent letters on the same line indicate statistical differences (P < 0.05)..Progesterone measurements with 91% sensibility. The coefficient of variation intra assay was 9.1 and 5.6% andbetween assays 2.4 and 0.6%.527.ReferencesMartins CM, Torres-Junior JRS, Gimenes LV, Souz~ AH, Baruselli PS. 2006. Acta Sci Vet, 34(supl. 1(abstract).Acknowlegments: F APESP: 06/55199-2, 07/57766-4 and EMBRAP A.E-mail: mateusjsudano@yahoo.com.br Re-utilization of ...2009 SP-PP-2009.0029(CPPSE- 18934-1268 p.268, Jan./Mar. 2009Anim. Reprod., v.6, n.PROCI.SUD2009SP-PP-2009.-2009.0029000290

Re-utilization of norgestomet implants in superovulated Nelore cows.

http://www.alice.cnptia.embrapa.br/bitstream/doc/48696/1/PROCI-2008.00193.pdf

Re-utilization of norgestomet implants in superovulated Nelore cows.

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