AZEVEDO, A. L. S.

CEZARIO R. R.

FAVERO, A. P.

OLIVEIRA, F. R.

VIGNA, B. B. Z.

Repository Open Access to Scientific Information from Embrapa

5th International Symposium of Forage Breeding – Buenos Aires, Argentina 2015   IDENTIFICATION OF DNA CONTENT OF DIFFERENT SPECIES OF Paspalum  Azevedo ALS1, Oliveira FR1, Cezário RR2, Vigna BBZ3, Fávero AP3.  1Embrapa Dairy Cattle, Juiz de Fora-MG, Brazil. 2Centro Universitário de Araraquara, UNIARA, Araraquara, SP, Brazil. 3Embrapa Southeast Livestock, São Carlos-SP, Brazil  Paspalum is a genus with many species of great potential for use as forage or turf. It is known as the most important genus in Gramineae family in America. The Germplasm Bank of Paspalum at Embrapa comprises about 350 accessions from 50 species, mainly from the informal group Plicatula. The objective of this study was to identify the DNA content of 300 accessions of Paspalum conserved to identify which may be diploid or tetraploid to finally adapt the best methodologies of conservation and use of germplasm. Leaves were collected in the field at Embrapa Southeast Livestock and wrapped in plastic bags with a small piece of moist paper towel and sent to Embrapa Dairy Cattle to perform the flow cytometry analysis. To determine the amount of DNA, approximately 20-30 mg of young leaf tissue for each sample, and the same amount of young leaf tissue of Raphanus sativus cv Saxa (internal reference standard with 1,11pg) were prepared. Nuclear suspension was added with propidium iodide and RNase. The analysis was performed on FACSCalibur cytometer, and the histograms were obtained in the Cell Quest software and analyzed in WinMDI software 2.8. From 47 evaluated species, the DNA content ranged from 1.32 pg for one accession of Paspalum modestum to 5.42 pg for an accession of P. pilosum. There was variation among accessions within species, and among species. Some accessions had already been confirmed, others still need confirmation to be classified as diploid or tetraploid.  Corresponding author: Alessandra Fávero, alessandra.favero@embrapa.br    

Identification of DNA content of different species of Paspalum.

http://www.alice.cnptia.embrapa.br/bitstream/doc/1037707/1/1-02-Azevedo-et-al.pdf

Identification of DNA content of different species of Paspalum.

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