BARBIERI, R. L.

FERRI, N. M. L.

HEIDEN, G.

LUCAS, J. V.

MAIA, L. C.

VAHL, B. R.

VIZZOTTO, M.

WAGNER, J. G.

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   Evaluation of bioactive compounds in two natural populations of Butia catarinensis Noblick & LorenziWAGNER¹, J.G .; FERRI2, N.M.L..; LUCAS1, J.V .; VAHL1, B.R.; MAIA³, L. C.; HEIDEN2, G.; VIZZOTTO, M2.; BARBIERI2, R.L¹Universidade Federal de Pelotas 1 - RS, goettenj@hotmail.com2Embrapa Clima Temperado 2 - RS, nubia.ferri@embrapa.br ¹Universidade Federal de Pelotas 3 – RS,  julianavolzlucas70@gmail.com ¹Universidade Federal de Pelotas 4 – RS, biavahl@gmail.com¹Universidade Federal de Pelotas 5 -  RS, lucianoc.maia@gmail.com ²Embrapa Clima Temperado 6 – RS,  gustavo.heiden@embrapa.br²Embrapa Clima Temperado 7 – RS, marcia.vizzotto@embrapa.br  ²Embrapa Clima Temperado 8 – RS, lia.barbieri@embrapa.br  Keywords: jelly palm; DPPH; caroten contentSummary:Butia is a genus of palms from South America, with an extensive history of use. Butia fruits are enjoyed for consumption in natura and incorporated into recipes. Large-scale natural populations of Butia species (palm groves), once common in southern Brazil, are now in decline due to land use changes and the low regenerative taxas. Among the 22 species of Butia, Butia catarinensis have its occurrence limited to the coastal areas of Santa Catarina and Rio Grande do Sul (Brazil), and is classified as an endangered species by official lists in these states. The aim of this work was to investigate wheter there are differences in the amount of carotenoids, total phenolic compounds, and antioxidant activity of two natural populations of B. catarinensis. Natural populations of B. catarinensis were addressed in march/2022 at Areais da Ribanceira, in Imbituba (SC/Brazil), and on a private property in Torres (RS/Brazil). Five bunches from different palms were collected in each population. The analyses were performed at the laboratory of Food Science and Technology from Embrapa Clima Temperado (Pelotas-RS), in triplicate. Total phenolic compounds was determined by Folin-Ciocolteu adapted method, by reading the absorbance at spectophotometer in wavelenght of 725 nm. Antioxidant activity was determined by DPPH assay, with absorbance reading at 515 nm. The methodology for quantifying carotenoid content was adapted from Talcot and Howard (1999), with wavelenght of 470 nm. Data was analyzed through Student’s T-Test (for independent samples) to compare the mean of the populations, Spearman correlation to distinguish the relations between the parameters, and Cluster Kmeans method to verify the genotypes distribution. All statistycal analysis were performed at R software. There was statistical difference among the populations for total phenolic content and antioxidant activity. The carotenoid content showed no difference between populations. The total phenolic content was expressed as a chlorogenic acid equivalents (CAE g/100g fresh weight), and the average in Imbituba’s population was 588.82 CAE g in 100g of fresh weight, statistically superior to Torres, whose average was 233.88. The antioxidant activity, expressed in µg trolox equivalent/g tissue, was also higher in Imbituba’s population, with an average of 1591.16 µg trolox equivalent/g tissue, while the average for Torres population was 373.41 µg trolox equivalent/g tissue. Average carotenoid content was 123.50 mg β-carotene equivalents/100g tissue in the population of Imbituba, and 136.16 in Torres. A strong positive correlation was found between total phenolic compounds and antioxidant activity (r = 0.878; p≤ 0.001), a result in consonance with the literature. No correlation was reported between carotenoid content and antioxidant activity, or total phenolic compounds. The clustering method showed the similarity of genotypes based on the place of origin by forming two groups, distinguishing the populations of Imbituba and Torres. It was possible discriminate the natural populations of B. catarinensis based on total phenolic content and antioxidant activity. Considering the role of environment conditions in bioactive expression, the quantification of these secondary metabolites in natural populations could provide relevant inferences about evolutionary responses and adaptative strategies in the different distribution areas of Butia.Enter the project / scholarship number (FAPESP, CAPES, CNPQ, etc.): We thank  the Ministry of Science, Technology and Innovations (MCTI) and CNPq for the financial support to Butia Palm Groves Netwoork, by process 441493/2017-3. . 

Evaluation of bioactive compounds in two natural populations of Butia catarinensis Noblick & Lorenzi.

http://www.alice.cnptia.embrapa.br/bitstream/doc/1152486/1/Trabalho-Campinas-Julia.pdf

Evaluation of bioactive compounds in two natural populations of Butia catarinensis Noblick & Lorenzi.

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