The objectives of this study were to determine the effect of calving to timed artificial insemination (C-TAI) interval on fertility of Bos taurus and Bos indicus cows and to determine the best C-TAI interval to include postpartum cows in TAI programs in each genotype (B. taurus and B. indicus). In experiment 1, a retrospective study was per-formed, with data from 2,709 TAI in Bos taurus and Bos indicus cows with different C- TAI intervals. There was a positive linear effect of C-TAI interval on the pregnancy probability (p 52 days). Cows with C-TAI longer than 52 days (C-TAI > 52) had greater pregnancy rate (56%) than cows with short C-TAI (46%; p .05) the ovulation rate nor the propor-tion of endometrial polymorphonuclear leucocytes. We conclude that C-TAI interval affects pregnancy rate in both Bos taurus and Bos indicus. We recommend a C-TAI in-terval longer than 52 days to increase fertility of beef cows included in TAI programs