Continuous ultraviolet (UV) irradiation stimulates the over-production of reactive oxygen species
(ROS) to cause degenerative diseases. Chlorogenic acid (CA) is found as plants antioxidant that
promises medicinal effects. This study examined CA protection against UV-damage in human skin
fibroblast (BJ) cells both for curative and preventive therapy. BJ cells were exposed to UV radiation and
the addition of CA (6.26-100 μg/mL) by preventive and curative addition methods. The cells viability
analysis was conducted employing MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-
2-(4-sulfophenyl)-2H-tetrazolium) assay. CA treatment before UV exposure exhibited an increased
percentage of viability cells than the positive control. In detail, the series of CA concentration (6.25,
12.5, and 25 μg/mL) significantly enhanced the percentage of viable cells. The addition of CA after UV
exposure denoted the same results. Furthermore, the lower CA concentrations used, the higher cell
viability resulted. CA at dose 6.25 μg/mL showed the highest viability in cells, while CA 100 μg/mL
resulted in the lowest viability. In short, CA can preserve and treat cells from UV exposure. The outcome
suggested prevention and curative on UV-induced BJ cells, and the tested concentration is applicable
for further experiments