Abstract The dissociation of normal human oxyhemoglobin has been studied by gel filtration under conditions of neutral pH and moderate ionic strength, with the use of both integral boundaries, formed between solution and solvent, and finite difference boundaries, formed between solution and solution. The experimental data obtained have been treated by nonlinear least squares procedures to estimate the relevant parameters with their associated standard errors. For this purpose, theoretical equations have been derived for two models, firstly a simple dimer-tetramer reversible equilibrium, and secondly a monomer-dimer-trimer-tetramer reversible equilibrium. In both models the dependence on concentration of the elution volume of the individual species has been taken into account
