Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI
Doi
Abstract
Gonadotropine Releasing Hormone (GnRH) is one of the recommended hormones to overcome ovulation problems and it can increase pregnancy rate so that it is used in government programs to increase cattle population in Indonesia, although the results are not yet optimal. Hormone and receptor compatibility is the main key to succesful hormone application while data on the composition of the Indonesian cow GnRHreceptors is not yet known. The purpose of this study was to obtain the composition of GnRH receptors cattle in Indonesia and compare them to the GenBank sequence reference so that is known whether genetic diversity occurs at GnRH receptors cattle in Indonesia. Samples in the form of 3 female cow hypothalamus stored at -800C temperatue freezer. The methods used are mRNA isolation, cDNA synthesis, implification of GnRH gene using Polymerase Chain Reaction (PCR) process by Promoter F and Exon 1 R primers and then elektrophoresis and sent for sequencing. Sequencing product were further aligned with the reference sequences of Bos TaurusGnRHR mRNA GenBank using the MEGA X program. The results of the analysis found the presence of Single Nucleotide Polymorphism (SNP) in the coding region of 1st cow position 38(A> T), 261(C > T), 342(C >T), 411(C >T) and 495(C > T) and 2nd cow positions 261(C >T). Changes in amino acids were also detected in 1stcowposition 13 (H> L). The presence of SNP was found to indicate genomic variation between individuals at GnRH receptors cattle in Indonesia
