Global challenges such as population growth, increasing urbanisation, climate change, and limited access to important plant nutrients have all contributed to the development of nanotechnology as a new innovation and its application in agriculture. Nanoparticles can be synthesized through chemical, physical or biological synthesis methods. However, the applied approach can significantly affect their chemical properties, reactivity and biological activity [1]. In recent years, the use of green synthesis methods has received increasing attention due to their ease of characterization, lower toxicity and favorable production costs. In our work, we examined and compared the species-dependent effects of CuO nanoparticles produced by both chemical and green synthesis on three different microgreen plants. Seed-priming was used for seed treatment and the biomass, pigment, and protein contents of one-week-old plants were investigated
