Institute for Genetic Engineering and Biotechnology, University of Sarajevo
Abstract
The binding of thyroid hormones to specific nuclear receptors in target cells induces synthesis of enzymes associated with redox processes, leading to the formation of reactive oxygen species (ROS), which can cause damage of DNA molecule. Quercetin has already been shown to have protective effect against DNA damage, with its most pronounced feature being scavenging of free radicals. The aim of this study was to evaluate antigenotoxic potential of quercetin against thyroxine-induced DNA damage in human whole blood cells by using the comet assay. For that purpose, cells were exposed to 50 μM thyroxine and separately pre-treated or post treated with 500 μM of quercetin. Results showed that DNA damage was significantly reduced in cells pre-treated with this scavenger of free radicals. Obtained results indicate the ability of thyroxine to be a mediator of DNA damage and that quercetin displayed protective effect against thyroxine-induced genotoxicity.1st Congress of Geneticists in Bosnia and Herzegovina with International Participation
Sarajevo, Bosnia and Herzegovina, 02nd-04th October, 2019, Book of abstract
