Anderson, Motswedi

Blackard, Jason

Essex, Max

Gaseitsiwe, Simani

Makhema, Joseph

Mohammed, Terence

Moyo, Sikhulile

Musonda, Rosemary

Sebunya, Theresa K

BMC Infectious Diseases

Harvard University - DASH 

 Prevalence and molecular characterization of Hepatitis B in HIVinfected individuals in Botswana  (Article begins on next page)The Harvard community has made this article openly available.Please share how this access benefits you. Your story matters.Citation Anderson, Motswedi, Simani Gaseitsiwe, Sikhulile Moyo,Terence Mohammed, Theresa K Sebunya, Jason Blackard,Joseph Makhema, Max Essex, and Rosemary Musonda. 2014.“Prevalence and molecular characterization of Hepatitis B inHIV infected individuals in Botswana.” BMC InfectiousDiseases 14 (Suppl 2): P86. doi:10.1186/1471-2334-14-S2-P86.http://dx.doi.org/10.1186/1471-2334-14-S2-P86.Published Version doi:10.1186/1471-2334-14-S2-P86Accessed February 17, 2015 5:41:57 AM ESTCitable Link http://nrs.harvard.edu/urn-3:HUL.InstRepos:13454667Terms of Use This article was downloaded from Harvard University's DASHrepository, and is made available under the terms and conditionsapplicable to Other Posted Material, as set forth athttp://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAAPOSTER PRESENTATION Open AccessPrevalence and molecular characterization ofHepatitis B in HIV infected individuals inBotswanaMotswedi Anderson1*, Simani Gaseitsiwe1, Sikhulile Moyo1, Terence Mohammed1, Theresa K Sebunya2,Jason Blackard3, Joseph Makhema1, Max Essex1,4, Rosemary Musonda1From International Symposium HIV and Emerging Infectious Diseases 2014Marseille, France. 21-23 May 2013IntroductionHepatitis B Virus (HBV) is a major coinfection in HIVinfected patients and it has emerged as an importantcause of morbidity and mortality in this group since thestart of Highly Active Antiretroviral Therapy (HAART).HIV/HBV coinfection prevalence varies by geographicr e g i o ne v e nw i t h i nt h es a m ec o u n t r y .I nB o t s w a n at h ec o i n f e c t i o np r e v a l e n c ed a t ai sv e r ys p a r s ea n dw h a ti sreported varies. Ten HBV genotypes with some subgeno-types have been described differing by geographic distri-bution, course of disease, response to treatment anddevelopment of mutations. Even though data have shownthat genotypes are predictive of disease outcome, the cir-culating HBV genotypes in Botswana remains unknown.Therefore this study aims at determining the HIV\HBVcoinfection prevalence rate and the circulating HBV gen-otypes in Botswana.Materials and methodsThis is a retrospective cross sectional study of HIV indivi-duals initiating HAART. Hepatitis B surface antigen(HBsAg) was screened for in 300 baseline samples andthose which were positive were also screened for HBeAg.For the HbsAg positive a 415 bp fragment of the HBVpolymerase gene was sequenced using Big Dye sequencingchemistry and the sequences were analyzed for genotypesand mutations.ResultsOf the 300 participants, 28(9.3%) were HBsAg positiveand 5(17.9%) were HBeAg positive. Of the 27 samplesthat were successfully genotyped, genotype A was foundin 23 (85.2%), 22 subgenotype A1 and 1 subgenotypeA2,genotype D subgenotype D3 made up the remaining 4(14.8%). Known escape mutations were found in 12(44.4%) with the most common one being N131N foundin 8(29.6%) of the participants. The other escape muta-tions were R122K, T123A, Q129R, G130N, M133T andF134V.No drug resistance mutations were detected.ConclusionThe HIV/HBV prevalence is consistent with what hasbeen reported in the country. The predominant genotypein this cohort is A1 and its consistent with findings fromthe region. Escape mutations previously associated withfailure of HBsAg detection and vaccine or immunoglo-blin therapy were found in a significant number of parti-cipants. No known drug resistance mutations were foundin the cohort.Authors’ details1Botswana Harvard AIDS Institute Partnership, Gaborone, Botswana.2University of Botswana, Department of Biological Sciences, Gaborone,Botswana.3University of Cincinnati College of Medicine, Cincinnati, USA.4Harvard School of Public Health, Department of Immunology and InfectiousDiseases, Boston, USA.Published: 23 May 2014doi:10.1186/1471-2334-14-S2-P86Cite this article as: Anderson et al.: Prevalence and molecularcharacterization of Hepatitis B in HIV infected individuals in Botswana.BMC Infectious Diseases 2014 14(Suppl 2):P86.1Botswana Harvard AIDS Institute Partnership, Gaborone, BotswanaFull list of author information is available at the end of the articleAnderson et al. BMC Infectious Diseases 2014, 14(Suppl 2):P86http://www.biomedcentral.com/1471-2334/14/S2/P86© 2014 Anderson et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the CreativeCommons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, andreproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Prevalence and molecular characterization of Hepatitis B in HIV infected individuals in Botswana

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Prevalence and molecular characterization of Hepatitis B in HIV infected individuals in Botswana

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