Sparrewath, Cillia Victoria Bech

With the ongoing rise in antimicrobial resistance, it is important to investigate possible alterna-tives to conventional antibiotics, such as antimicrobial peptides and peptidomimetics, such as peptoids. Peptoid nanosheets are self-assembling, free floating 2D structures that can be function-alised in a variety of ways. Peptoids were synthesised for the formation of functionalised nanosheets with an antimicrobial functional element. Nanosheet formation combining loop-less and loop-holding peptoids were investigated and the resulting nanosheets were imaged and char-acterised by optical and atomic force microscopy. The nanosheets were tested against E. coli in a MIC test and the bacterial growth was monitored. Synthesis of short GN-2 analogue peptoids with lipid-tails were successful, while it was not possi-ble to block peptoids with GN-2 peptide and peptoid loops. The combined nanosheets formed plenty ordered nanosheets, though not to the degree of B28. AFM measurements of nanosheets were seen to not be in accordance with the literature, as B28 was thicker and rougher, while the combined nanosheets were thinner and less rough than expected. The presence of unprotected het-erocyclic side chains due to the amount of tryptamines continue to be a challenge for the synthesis of block peptoids. It was difficult to evaluate the composition of the combined nanosheets based on the AFM measurements, which made it difficult to know what to expect from the nanosheets in the biological assays.<br/

Functionalised peptoid nanosheets: Synthesis, formation, characterization

Abstract

Similar works

Full text

Available Versions

Roskilde Universitet