Abstract— The implementation of Controller Area Network (CAN) in automotive testing and development processes often involves the use of licensed interfaces to communicate between computers and Electronic Control Units (ECUs). The main objective of this work is to present a cost-effective alternative to the Universal Serial Bus (USB) protocol interfaces currently available in the market. An iterative Printed Circuit Board (PCB) design is integrated with bespoke software, similar to CANoe and VehicleSPY, in terms of testing development. The results demonstrate that an in-house customized, upgradable, cost-effective USB-CAN interface bridges the gap with the software reducing the dependence on licensed interfaces. This research contributes to the advancement of hardware development for USB-CAN interfaces and showcases an affordable option for automotive software testing and development processes.ITESO, A. C