Bassin, João Paulo

Castro, Paula M. L.

Dezotti, Márcia W. C.

Ely, Cyntia

Gonçalves, David M.

Moreira, Irina S.

English

Repositório Institucional da Universidade Católica Portuguesa

Bacterial strain Rhodococcus sp. ED55 degrades endocrine disrupring chemicals in wastewaterIrina S. Moreira 1, Cyntia Ely 2, João Paulo Bassin 2, Márcia W. C. Dezotti 2, David M. Gonçalves 3, Paula M.L. Castro 11 Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola                                                                                    Superior de Biotecnologia, Portugal2 Federal University of Rio de Janeiro, Brazil3 Institute of Science and Environment, University of Saint Joseph, Macau S.A.R., ChinaIntroduction Isolation of Rhodococcus sp. ED5517β-estradiol biodegradation by Rhodococcus sp. ED55ConclusionsAcknowledgementsEndocrine disrupting chemicals (EDCs) is a group of chemicals that can alter thehormonal and homeostatic systems of organisms. An increasing concern onpossible environmental and human health effects is due to evidence of negativeeffects as a result of exposure to very low concentrations [1]. There is a long listof EDCs, which include natural and synthetic estrogens and industrial chemicalssuch as bisphenol A. It is commonly accepted that the major source of EDCs tothe environment is wastewater treatment plants (WWTPs)’ effluents, due to theirinefficiency to remove this kind of pollutants [2]. Bioaugmentation, the process ofadding selected bacterial strains to improve degradation of recalcitrantcompounds, is a promising technique to improve removal of pollutants in WWTP.A bacterial strain, Rhodococcus sp. ED55, isolated from WWTP in Coloane (Macao),for its ability to degrade EDCs, can potentially be applied in bioaugmentationstrategies for ameliorating treatment of wastewater contaminated with EDCs.➢A bacterial strain identified as Rhodococcus sp. ED55 was isolated from thesediments of discharge point of WWTP located in Macao;➢Rhodococcus sp. ED55 was able to degrade E2 in synthetic medium and in realwastewater;➢Bioaugmentation with strain ED55 improved natural E2 attenuation in realwastewater;➢Biodegradation is accompanied by removal of toxicity and decrease of estrogenicity;➢Bioaugmentation of an AGS reactor with strain ED55 improved biodegradation ofBPA.AGS bioaugmented with Rhodococcus sp. ED55✔ Complete degradation of E2 in few hours as only carbon sourceIn real wastewaterColleted from the primary settling tank of a municipal WWTP (Parada, Maia – Portugal)0.01.02.03.04.05.06.00 2 4 6 8 10 12 14 16 18 20 22 24E2 concentration (mg L-1)Time (hours)E2 + ED55E2E2 + ED55 sterile WWIn synthetic salts medium✓Bioaugmentation with strain ED55improved the natural attenuation of E2 inreal wastewaterEvaluation of whole sample toxicity – Bioluminescence inhibition (%)WW sterile WW not sterileBefore degradation assay 55,5 50,2After (without ED55) 49,2 12,6After (with ED55) 2,3 0WW sterile WW not sterile MMBefore degradation assay > 400 > 400 352,2After (without ED55) > 400 < LOD > 400After (with ED55) 126,3 < LOD 124,9Evaluation of whole sample estrogenecity – S-YES assay (EEQ)Elimination of the toxicity of the treated effluentDecrease in the estrogenic activity after biodegradationSynthetic saline wastewater +17β–estradiol (E2), 17α–ethinylestradiol (EE2) and bisphenol-A (BPA)+Bioaugmentation with Rhodococcus sp. ED55 (phase VII)✓BPA biodegradation 51 – 95%  Degrading strain(phase VII)✓EE2 adsorption/desorption✓E2 biodegradation 100%InfluentAfter anaerobic feeding – theoreticalAfter anaerobic feeding - measured massEffluent(•) Anaerobic removal(○) Removal under aerationImproved after bioaugmentationCyntia Ely was financed by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001. This work was supported by National Funds from FCT - Fundação para a Ciência e a Tecnologia - through the project AGeNT - PTDC/BTA-BTA/31264/2017 (POCI-01-0145-FEDER-031264). We would also like to thank the scientific collaboration of CBQF under the FCT project UID/Multi/UIDB/50016/2020.ReferencesAerobic granular sludge (AGS) is a technology now largely adopted in WWTP➢ The natural hormone,17β-estradiol (E2) was chosen as model compound to study biodegradation of EDCs by strain ED55 [4]➢ Strain ED55 was used in a bioreactor to treat wastewater containing EDCs [5][1] Xu G. et al. (2016). Environ. Sci. Pollut. Res. 25442–25450. doi:10.1007/s11356-016-7669-y.[2] Thorpe K.L. et al. (2003). Environ. Sci. Technol. 37, 1142–1149. doi:10.1021/es0201348.[3] Moreira  I.S. et al. (2021). Biodegradation 32, 519-529. doi:10.1007/s10532-021-09948-9. [4] Moreira  I.S. et al. (2022). Int. J. Mol. Sci. 23, 6181. doi:10.3390/ijms23116181.[5] Ely C. et al. (2022). J. Environ. Chem. Eng. 10, 107272. doi:10.1016/j.jece.2022.107272.Plating with target compoundSediments Colonies of degrading bacteriaTarget compoundBacterial identification16S rRNA gene sequencing Target compoundBiodegradation by the bacterial isolates⮚Bisphenol A (BPA)⮚Bisphenol S (BPS)⮚17β-estradiol (E2)⮚17α-ethynilestradiol (EE2)➢ Isolation from sediments near the discharge point of Coloane WWTP, Macao [3]➢ Evaluation of toxicity before and after E2 biodegradation by strain ED55 

Bacterial strain rhodococcus sp. ED55 degrades endocrine disrupring chemicals in wastewater

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Bacterial strain rhodococcus sp. ED55 degrades endocrine disrupring chemicals in wastewater

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