Carla Andrea Alonso

Carmen Torres

Jean-Yves Madec

Marisa Haenni

Myriam Zarazaga

Pauline François

Pierre Châtre

Sandra Martínez-Álvarez

Teresa Cardona-Cabrera

Úrsula Höfle

English

Repositorio de Universidad de La Rioja

                 2023 JULY 3-5 TOURS FRANCE   9th	Symposium	on		Antimicrobial	Resistance	in	Animals	and	the	Environment	Abstracts	book	PALAIS DES CONGRÈS TOURS FRANCE  41 Speaker Abstracts   O27  High occurrence of carbapenemase- and extended-spectrum-β-lactamase-producing Escherichia coli and Klebsiella pneumoniae from migratory birds (Ciconia ciconia) with detection of high-risk clones  Sandra MARTÍNEZ-ÁLVAREZ1 Pierre CHÂTRE2, Úrsula HÖFLE3, Carla Andrea ALONSO4, Pauline FRANÇOIS2, Teresa CARDONA-CABRERA2, Myriam ZARAZAGA1, Jean-Yves MADEC2, Marisa HAENNI2, Carmen TORRES1  1Area of Biochemistry and Molecular Biology, OneHealth-UR Research Group, University of La Rioja - Logroño (Spain) 2ANSES – Université de Lyon, Unité Antibiorésitance et Virulence Bactériennes - Lyon (France) 3Health and Biotechnology (SaBio) Research Group, Institute for Game and  Wildlife Research IREC (CSIC-UCLM) - Ciudad Real (Spain) 4Hospital San Pedro, Logroño (Spain)  Background: The presence of Enterobacterales producing carbapenemases (CP) and extended-spectrum β-lactamases (ESBL) is closely monitored in humans and animals, but the potential of migratory birds as carriers of resistance genes remains poorly understood. The aim of the study was to detect and characterize CP- and ESBL-producing E. coli (EC) and K. pneumoniae (KP) obtained from storks feeding on two landfills in Spain.  Methods: ESBL and CP-producing EC/KP were isolated from 211 stork faecal samples using chromogenic culture media, and collected isolates were sequenced using NovaSeq6000 (Illumina) and MinION (Oxford Nanopore) technologies. Resistome, virulome, sequence types (ST) and replicon profiles were determined using bioinformatic tools. Localization of ESBL/CP genes was performed using Southern blots on S1-PFGE gels.   Results: ESBL-EC/KP were detected in 71 samples (33.6%; 28.4%-EC and 5.2%-KP), while 28 samples (13.3%) contained CP-producing EC/KP (11.8%-EC and 1.4%-KP). Different sequence types (ST) (EC, n=33; KP, n=3) were identified, including high-risk clones associated with humans (EC: ST131, ST58 or ST69; KP: ST307), potential high-risk clones (EC: ST10 and ST48) and more ubiquitous clones (EC: ST46, ST155, ST117, ST617). A wide range of ESBL/pAmpC-conferring genes (blaCTX-M-1/blaCTX-M-14/blaCTX-M-15/blaCTX-M-27/blaCTX-M-32/blaCTX-M-55/blaCTX-M-65/blaSHV-12/blaCMY-2/blaDHA-1) was identified, as well as a large variety of CP-genes (blaKPC-2, blaKPC-3, blaNDM-1, blaNDM-7, blaOXA-48, blaVIM-1 and blaGES-7), including in some cases a combination of up to three types of CP (blaKPC-2, blaNDM-7 and blaVIM-1) and/or with co-detection of ESBL/pAmpC genes. Likewise, a variety of ESBL/pAmpC- and CP-carrying plasmids were identified, such as IncY (n=30), IncF (n=60), IncX3 (n=25) IncN (n=19) or IncL (n=8). The genetic characterization of these plasmids is ongoing.     

High occurrence of carbapenemase- and extended-spectrum-β-lactamase-producing Escherichia coli and Klebsiella pneumoniae from migratory birds (Ciconia ciconia) with detection of high-risk clones

https://investigacion.unirioja.es/documentos/64abdaf1cc8ad211a9592d0c/f/64ad4239cc8ad211a9592db4.pdf

High occurrence of carbapenemase- and extended-spectrum-β-lactamase-producing Escherichia coli and Klebsiella pneumoniae from migratory birds (Ciconia ciconia) with detection of high-risk clones

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