Carmen Torres

Haythem Gharsa

Houssem Ben Yahia

Idris Nasir Abdullahi

Karim Ben Slama

Maha Hammami

Rabeb Issaoui

Sandra Martínez Álvarez

English

Repositorio de Universidad de La Rioja

  September 22-24 2022, The Royal Hotel, Hammamet, Tunisia                      THIRD INTERNATIONAL SYMPOSIUM ON NATURAL ANTIMICROBIALS:  Current status, challenges and perspectives  Book of abstracts  47  September 22-24 2022, The Royal Hotel, Hammamet, Tunisia ISOLATION AND CHARACTERIZATION OF LACTOBACILLUS REUTERI FROM AVIAN CECAL CONTENT Rabeb Issaoui1, Houssem Ben Yahia 1,2, Idris Nasir Abdullahi3, Sandra Martínez Álvarez3, Haythem Gharsa 1,2, Maha Hammami1, Carmen Torres3, Karim Ben Slama1,2 1 Institut Supérieur des Sciences Biologiques Appliquées de Tunis, Université de Tunis El Manar, 1006 Tunis, Tunisia 2 Laboratoire des Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université Tunis El Manar, 2092 Tunis, Tunisia 3 Area de Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, Spain. Background: In developing countries, the poultry agro-business sector has largely been floated with indiscriminate use of antimicrobial agents to enhance production. These could consequently encourage the re-emergence and transmission of antimicrobial resistant bacteria to humans. Objective: This study aims to characterize the antimicrobial resistance profile of Lactobacillus reuteri obtained from avian species, isolate, and identify biochemical alternatives that could be useful as antibiotics. Methodology: One hundred six cecal contents of chicken from different regions of Northern Tunisia were collected, processed (February-September 2021) and Lactobacillus reuteri strains were isolated from the Man-Rogosa-Sharpe (MRS) medium. Identification of isolates was carried out by PCR and MALDI-TOF-MS. Antimicrobial susceptibility phenotypes and corresponding genes of all L. reuteri isolates were analyzed by disk diffusion and PCR, respectively. The evaluation of the antimicrobial activity was performed by direct antagonistic test on various indicator bacteria. Results: Seventy-six isolates were identified as Lactobacillus spp by PCR. Further analysis by MALDI-TOF-MS revealed that 4 bacteria (12,12 %) out of 33 strains tested show scores of strong similarities to L. reuteri. All the L. reuteri isolates were susceptible to penicillin and clindamycin. These strains were resistant to erythromycin, gentamycin, sulfamethoxazole-trimethoprim, and ciprofloxacin. Strains X4392, X4393 and X4394 were resistant to tetracycline, vancomycin and tobramycin. For chloramphenicol, these three bacteria exhibit intermediate resistance phenotype. As for the strain X4395, it was susceptible to clindamycin, tobramycin and vancomycin. All the L. reuteri strains harbored the erm(B) gene, but lacked the erm(A), erm(C), erm(T), aac(6´)-aph(2”), fex(A) and fex(B) genes. The strain X4395 carried tet(L), tet(M) and tet(K) genes. However, only tet(L) was detected in strains X4392 and X4394. Also, all strains have significant antagonistic effects against several indicator bacteria such as S. aureus and Enterococcus spp. Conclusion: These data indicate that L. reuteri that is supposedly a ‘safe’ probiotic could acquire AMR determinants of human health concerns. It could be inferred that L. reuteri presents ‘double-edged sword’ traits, as it presents itself as a reservoir of AMR genes and contains biomolecules that can be useful to fight against some pathogenic bacteria.  

Isolation and characterization of lactobacillus reuteri from avian cecal content

https://investigacion.unirioja.es/documentos/646b8c5d325e920f4d27ad9c/f/646c98767bb1586d2f0537ea.pdf

Isolation and characterization of lactobacillus reuteri from avian cecal content

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