Grundmeier, Guido

Keller, Adrian

Kollmann, Fabian

Kostiainen, Mauri A.

Linko, Veikko

Ramakrishnan, Saminathan

Shen, Boxuan

English

Aaltodoc Publication Archive

Superstructure-Dependent Loading of DNA Origami Nanostructures with a Groove-Binding Drug

DNA
origami nanostructures are regarded as powerful and versatile
vehicles for targeted drug delivery. So far, DNA origami-based drug
delivery strategies mostly use intercalation of the therapeutic molecules
between the base pairs of the DNA origami’s double helices
for drug loading. The binding of nonintercalating drugs to DNA origami
nanostructures, however, is less studied. Therefore, in this work,
we investigate the interaction of the drug methylene blue (MB) with
different DNA origami nanostructures under conditions that result
in minor groove binding. We observe a noticeable effect of DNA origami
superstructure on the binding affinity of MB. In particular, non-B
topologies as for instance found in designs using the square lattice
with 10.67 bp/turn may result in reduced binding affinity because
groove binding efficiency depends on groove dimensions. Also, mechanically
flexible DNA origami shapes that are prone to structural fluctuations
may exhibit reduced groove binding, even though they are based on
the honeycomb lattice with 10.5 bp/turn. This can be attributed to
the induction of transient over- and underwound DNA topologies by
thermal fluctuations. These issues should thus be considered when
designing DNA origami nanostructures for drug delivery applications
that employ groove-binding drugs

Fabian Kollmann (5644607)

Saminathan Ramakrishnan (3308727)

Boxuan Shen (3211524)

Guido Grundmeier (2088949)

Mauri A. Kostiainen (1425055)

Veikko Linko (1824070)

Adrian Keller (1303593)

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Superstructure-Dependent Loading of DNA Origami Nanostructures
with a Groove-Binding Drug

https://figshare.com/articles/Superstructure-Dependent_Loading_of_DNA_Origami_Nanostructures_with_a_Groove-Binding_Drug/6984056

Superstructure-Dependent Loading of DNA Origami Nanostructures with a Groove-Binding Drug

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