FUKASAWA, Yu

ISHII, Kaho

English

Tohoku University Repository (TOUR) / 東北大学機関リポジトリ

24PosterEffects of the Resource Distance on the Decisions of Mycelial BehaviorKaho ISHII1 and Yu FUKASAWA21Faculty of Agriculture, Tohoku University, Japan2Graduate School of Agricultural Science, Tohoku University, JapanCord-forming basidiomycetes develop large mycelial networks and play a major role in organic matter decomposition and nutrient cycling in forest ecosystems. Fungal mycelia alter their foraging and migrating behavior fl exibly in response to the presence of outer resource and their nutritional status. Previous studies showed that, when a mycelium extending from a wood block (inoculum) fi nds a new piece of wood (bait), the mycelium makes a decision whether or not to move from the inoculum to the bait depending on the bait size and the timing of bait placement. For example, relatively larger baits tend to induce mycelial migration from inoculum wood to bait wood more frequently than smaller ones. This suggests that mycelia may decide to move out based on the size of the wood resources and their own nutritional status. However, the infl uence of other factors, such as distance from the inoculum to the bait, on the decision to move out is not clear. We hypothesized that, if the distance from the inoculum is longer, mycelia might move to the baits even with smaller size, because maintaining the hyphal connection between the inoculum and the bait at a longer distance might be energetically more expensive than that in a shorter distance. Therefore, this study examined the eff ects of distance from the inoculum to the bait and the size of the bait on the decision-making of mycelial migration.A beech wood block (0.5 cm3), incubated with a basidiomycete Phanerochate veltina for 1 month on 2% malt extract agar medium for colonization, was put onto a corner of a tray (24 cm × 24 cm) fi lled with soil ca. 5 mm depth (Fig. 1). When mycelium grew at least 15 cm from the inoculum, a bait wood block was placed 1 cm or 15 cm away from the inoculum and incubated at 20˚C in the darkness for 50 days. We prepared two sizes of bait wood blocks (4 cm3 and 8 cm3). After incubation, inoculum wood blocks were retrieved from the tray, surface cleaned, transferred to new soil trays, and incubated at 20˚C in the darkness for 8 days to check the re-growth of mycelium and its direction. Trays were photographed every 3 days during the experiment. We measured hyphal coverage by ImageJ. Inoculums and baits were dried at 70˚C and weighed before and after incubation to check their weight loss during incubation. The dry weight of 40 inoculums at the time of bait placement was estimated by creating a regression line from the dry weight and mycelial area of 73 inoculums removed at that time.Poster 8Figure. Experimental design of microcosm

Effects of the Resource Distance on the Decisions of Mycelial Behavior

https://tohoku.repo.nii.ac.jp/record/2000019/files/JIFS Vol20 - P24 Poster8.pdf

Effects of the Resource Distance on the Decisions of Mycelial Behavior

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