The data set contains relevant information for the associated manuscript "Automated Purification of DNA Origami with SPRI Beads". It contains the Supporting Information 2, the origami design file and AFM images used in the publication

Actis, Paolo

Chau, Chalmers

Macaulay, Iain

Mohanan, Gayathri

Wälti, Christoph

Research Data Leeds Repository (University of Leeds)

1  Supporting Information 2 for:  Automated Purification of DNA Origami with SPRI Beads Chalmers Chau1,2 *ǂ, Gayathri Mohanan1,2ǂ, Iain Macaulay3,4, Paolo Actis1,2, Christoph Wälti1,2 * 1 School of Electronic and Electrical Engineering, University of Leeds, LS2 9JT, UK 2 Bragg Centre for Materials Research, University of Leeds, LS2 9JT, UK 3 Earlham Institute, Norwich Research Park, Norwich, NR1 7UZ, UK 4 School of Biological Sciences, University of East Anglia, Norwich, Norfolk, NR4 7TJ, UK ǂ These authors contribute equally. * Corresponding: c.c.chau@leeds.ac.uk; c.walti@leeds.ac.uk;    2  Intact origami counting Throughout this section, for origamis that are counted as intact, a black number will be used. For origamis that are counted as damaged, a red number will be used. Below shows an AFM image as an example:  In this example, a total of 17 intact origamis and 4 damaged origamis.   3  S-400 HR Images below were used to quantify the number of intact origamis purified using S-400 HR spin column.   4      5  SPRI – 0.8X ratio Images below were used to quantify the number of intact origamis purified using SPRI at 0.8X ratio column.   6    7      8  MWCO – 1 Images below were used to quantify the number of intact origamis purified using 100 kDa MWCO – 1 concentrator.   9       10  MWCO – 2 Images below were used to quantify the number of intact origamis purified using 100 kDa MWCO – 2 concentrator.     11  Ethanol Precipitation Images below were used to quantify the number of intact origamis purified using ethanol precipitation.  12     13      14  PEG precipitation Images below were used to quantify the number of intact origamis purified using PEG precipitation.   15     16     17  Phase separation Images below were used to quantify the number of intact origamis purified using phase separation method.   18      19  Size Exclusion Chromatography Images below were used to quantify the number of intact origamis purified using size exclusion chromatography.   20     21      22  Intact CRP functionalised DNA origami count Throughout this document, for origamis that are counted as intact, a green circle will be used. For origamis that are counted as damaged, a red circle will be used. For origamis that are intact but no CRP, a blue circle will be used. Below shows an AFM image as an example:  In this example, a total of 11 intact origamis with CRP, 9 intact origamis and 10 damaged origamis.    23  SPRI cleaned DNA origamis   24    25    

Dataset for Automated Purification of DNA Origami with SPRI Beads

https://archive.researchdata.leeds.ac.uk/1190/3/supporting_information_2.pdf

Dataset for Automated Purification of DNA Origami with SPRI Beads

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Research Data Leeds Repository (University of Leeds)