EL SOURY, MARWA MOHAMED EMADELDIN ALI MAHMOUD

Metafune, Miriam

Perroteau, Isabelle

Raimondo, Stefania

English

Institutional Research Information System University of Turin

In vitro Characterization Of Different DecellularizationProtocols for Peripheral Nerve Grafts Preparation.Poster No:1496 Authors:Marwa El Soury , Óscar Darío García-García , Miriam Metafune , Matteo Moretti , IsabellePerroteau , Stefania Raimondo , Arrianna Barbara Lovati , Victor CarrielInstitutions:Neuroscience Institute Cavalieri Ottolenghi (NICO), Torino, Torino, Nerve regeneration Group,Department of Clinical and Biological Sciences, University of Torino, Torino, Italy, TissueEngineering Group,Department of Histology, University of Granada, Granada, Spain, Instituto deInvestigacion Biosanitaria, Ibs.GRANADA, Granada, Spain, Dipartment of Clinical and biologicalsciences, Orbassano, Torino, IRCCS Istituto Ortopedico Galeazzi, Cell and Tissue EngineeringLaboratory, Milan, Italy, Neuroscience Institute Cavalieri Ottolenghi (NICO), Orbassano, Italy,Tissue Engineering Group,Department of Histology, University of Granada., Granada, Granada,Instituto de Investigacion Biosanitaria, Ibs.GRANADA, Granada, Spain, Granada, SpainIntroduction:In critical nerve gaps, where tensionless repair is unapplicable, a nerve graft or conduit areneeded to connect the two nerve stumps. Decellularized nerve allografts are a promising tissueengineering strategy. Their superiority over nerve conduits is owed to the availability of naturalwell-conserved extracellular matrix (ECM) components that has proven to play an important rolein supporting axonal guiding and peripheral nerve regeneration.The known decellularization techniques nowadays are time and effort consuming. In the presentwork performed on rat sciatic nerves, we investigated a novel nerve decellularization protocolable to combine an effective decellularization in short time with a good ECM preservation.Methods:Two different decellularization protocols were tested. The first protocol - proven to be efficient fordecellularizing tendons (DN-P1), using 1% tri(n-butyl) phosphate (TBP), 3% peracetic acid (PAA)(Lovati et al., 2016) - was compared with a decellularization protocol specifically developed fornerves (DN-P2), using 125mM SB-10, 0.2% TritonX-100, 0.25% SDS, and sonification cycles(Boriani et al.; 2017). The outcomes of both decellularization protocols were assessed by a seriesof in vitro evaluations, including qualitative and quantitative histological andimmunohistochemical analyses, DNA quantification, SEM and TEM ultrastructural analyses,mechanical testing, and viability assay.Results:Both decellularization protocols had led to an overall well-preserved nerve structure; DNAquantification showed that DNA content was significantly decreased, but not completely removed.1,2 3,4 5 65,7 5,7 6 8,91 23456789PNS https://ww6.aievolution.com/pns2301/index.cfm?do=abs.viewAbs&ab...1 of 3 1/16/2023, 9:30 AMBoth protocols had less cellular component, but complete removal was not achieved; an adequateamount of ECM component is still conserved in both protocols. DN-P1 has better biomechanicalproperties, superior biocompatibility and ultrastructural properties compared to DN-P2.Conclusions:DN-P1 greatly demonstrated superior results compared to DN-P2 in terms of ultrastructuralanalysis and biocompatibility. Decellularized nerve allografts prepared following DN-P1 protocolare promising for long gap repair in vivo.Special Interest Group:Neuropathic Pain Consortium (NPC)Biology:AxonClinical:N/ATechniques:ImagingAdditional InformationThe abstract submitted reports the results of a Clinical Study (i.e., a prospectivestudy of a new test or treatment in humans)NoDoes the abstract submitted primarily report on basic science research?YesHas your abstract previously been presented at a national/international meeting?YesIf yes, please cite the meeting:93 th congress SIBS “Società Italiana di Biologia Sperimentale “, Palermo (online), Italy.Was this abstract previously presented at the 2022 PNS Annual Meeting in Miami,Florida, USA?NoFieldScientistPNS https://ww6.aievolution.com/pns2301/index.cfm?do=abs.viewAbs&ab...2 of 3 1/16/2023, 9:30 AMAuthor ApprovalPlease confirm your plans for attendance at the PNS Annual Meeting in Copenhagen.I plan to attend the PNS Annual Meeting in Copenhagen. I understand that there is no option foran e-poster.KeywordsKeyword 1Nerve AllograftKeyword 2DecellularizationKeyword 3Nerve RepairPrizesMembership Status Note: Junior PNS Members, please click here(https://account.pnsociety.com/i4a/ams/public/member_start.cfm?mbrAppID=1&pageID=3516) to view the membership criteria.Non-MemberReferencesI have references I would like to disclose.NoTravel GrantsWould you like to apply for a PNS Travel Grant?NoPNS https://ww6.aievolution.com/pns2301/index.cfm?do=abs.viewAbs&ab...3 of 3 1/16/2023, 9:30 AM

In vitro Characterization of Different Decellularization Protocols for Peripheral Nerve Grafts Preparation

https://iris.unito.it/bitstream/2318/1918794/1/PNS%20Abstract%202023.pdf

In vitro Characterization of Different Decellularization Protocols for Peripheral Nerve Grafts Preparation

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