Alessandria, Valentina

Botta, Cristian

Buzzanca, Davide

Cocolin, Luca. S.

Rantsiou, Kalliopi

English

Institutional Research Information System University of Turin

17 October 2023AperTO - Archivio Istituzionale Open Access dell'Università di TorinoOriginal Citation:Evaluation of Aliarcobacter butzleri transcriptome during simulated infection of a humanintestinal modelTerms of use:Open Access(Article begins on next page)Anyone can freely access the full text of works made available as "Open Access". Works made availableunder a Creative Commons license can be used according to the terms and conditions of said license. Useof all other works requires consent of the right holder (author or publisher) if not exempted from copyrightprotection by the applicable law.Availability:This is the author's manuscriptThis version is available http://hdl.handle.net/2318/1830250 since 2022-01-04T18:03:05ZAliarcobacter butzleri is a Gram negative bacterium of the Campylobacteraceae family often isolated from fecal samples from different animals and human clinical cases. In the last years, they are increasingly being isolated from different types of meats (in particular pork, chicken) and have been associated with symptoms such as diarrhea, vomiting, abdominal pain in humans. This study led to evaluate the invasion and colonization ability of 3 A. butzleri strains isolated from human on in vitro intestinal cell models obtaining transcriptomic data of A. butzleri in virulence conditions. The RNAseq analysis of the strains LMG10828T, LMG11119 and 31 has been performed with the use of a mixed mucus producer cell models (Caco-2 + HT29 MTX, 9/1 ratio) at 30 and 90 minutes from the inoculum jointly at an evaluation of the colonization/invasion ability of the strains. The RNA obtained has been sequenced with Illumina technology and the number of reads, mapped on the gene sequences, have been elaborated with the software edgeR to allowing the determination of differentially expressed genes (DEGs). The data shown greater expression of genes related to TonB protein (intracellular growth), membrane protein and oxidoreductase activity (energy supply) in conditions of virulence in the strains tested. Moreover, the Inner membrane protein YjcH gene results overexpressed at 30 and 90 minutes from the inoculum, jointly with the gene codify for Cation/acetate symporter ActP at 90 minutes. Genes related to tonB function, exbB and exdB result overexpressed at 90 minutes together only in the strain LMG 11119 (greater colonization than the other two strains, p < 0.05), resulting not DEGs or underexpressed in the strains 31. Moreover, recently the urease pathway has been linked to the A. butzleri virulence, a part of these genes result underexpressed in the strains 31 that showed a lower colonization.  

Evaluation of Aliarcobacter butzleri transcriptome during simulated infection of a human intestinal model

https://iris.unito.it/bitstream/2318/1830250/1/abstract%20MD%202021%20D%20Buzzanca.pdf

Evaluation of Aliarcobacter butzleri transcriptome during simulated infection of a human intestinal model

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