Bernardini, M.

Brossa, A.

Bussolati, B.

Chinigò, Giorgia.

Genova, T.

Gkika, D.

Munaron, L.

English

Institutional Research Information System University of Turin

16 October 2023AperTO - Archivio Istituzionale Open Access dell'Università di TorinoOriginal Citation:TRP EXPRESSION SIGNATURE IN TUMOR-DERIVED ENDOTHELIAL CELLS: FUNCTIONAL ROLES INPROSTATE CANCER ANGIOGENESISTerms of use:Open Access(Article begins on next page)Anyone can freely access the full text of works made available as "Open Access". Works made availableunder a Creative Commons license can be used according to the terms and conditions of said license. Useof all other works requires consent of the right holder (author or publisher) if not exempted from copyrightprotection by the applicable law.Availability:This is the author's manuscriptThis version is available http://hdl.handle.net/2318/1801564 since 2021-09-15T12:31:27ZTRP expression signature in tumor-derived endothelial cells: functional roles in prostate cancer angiogenesisMichela Bernardini1,2,3, Giorgia Chinigò1,2 Guillaume Grolez2,3, Alessia Brossa4, Giulia Trimaglio2,3, Laurent Allart2,3, Audrey Hulot7, Guillemette Marot7,8,  Tullio Genova1, Aditi Joshi1, Virginie Mattot6, Gaelle Fromont-Hankard5, Fabrice Soncin6, Benedetta Bussolati4, Natalia Prevarskaya1,2, Luca Munaron1, Alessandra Fiorio Pla1,2,3 & Dimitra Gkika1,21Department of Life Science and Systems Biology, University of Torino, Turin, Italy. 2Inserm U1003, Université Lille 1, Villeneuve d’Ascq, France. 3Laboratory of Excellence, Ion Channels Science and Therapeutics, Université de Lille 1,Villeneuve d’Ascq, France. 4Department of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Centre, University of Torino, Turin, Italy. 5 Inserm UMR 1069, Université de Tours, Tours, France. 6Institute deBiologie de Lille, Lille, France. 7Univ. Lille, Institut Français de Bioinformatique, bilille, F-59000 Lille, France. 8Univ. Lille, Inria, CHU Lille, EA 2694-MODAL-Models for Data Analysis and Learning, F-59000 Lille, France.It was previously shown that PTEC exhibit the aggressive phenotype typical of TECs (Fiorio Pla et al. 2014). Here we identified three ‘prostate specific’ TECoverexpressed TRPs: TRPV2, TRPC3 and TRPA1 involved in different aspects of the angiogenic process. Taken together, our expression profiling and functional data could explain thetransition of prostate endothelial cells to their aggressive tumor phenotype, proposing novel molecular players to selectively target PCa progression and angiogenesis. Results recentlypublished (Bernardini et al., Cancers 2019).‘ Prostate-specific’ TRPV2 enhances EC proliferationTRP channels play a key role in cancer progression, modulating cell proliferation and survival, cancer invasion of surrounding tissues and angiogenesis. TRPexpression could therefore characterize the prostate cancer (PCa) cell phenotype. Another well-established concept is that TRPs deeply modulate endothelial cell (EC) biology andtumor angiogenesis. However, a specific TRP expression signature of PCa angiogenesis is still lacking.Our aim was to profile the expression of TRP channels during PCa angiogenesis and then to identify the specific molecular modulators of this process proving novel therapeutic targets.TRP channel expression profile in normal and tumor-derived EC highlights a ‘Prostate specific’ pattern‘ Prostate-specific’ TRPA1 enhances EC migrationRandom migrationassayCa2+ imagingCa2+ imagingCell viabilityassay‘ Prostate-specific’ TRPC3 promotes Pcacell attractionCa2+ imagingTRPA1 promotes EC tubulogenesis in vitro and angiogenesis in vivoTRPA1 is implicated in PTEC chemotaxis and function in tip-like cells ImmunostainingCONCLUSIONINTRODUCTIONhTERT PTECTRPA1 TRPV2 TRPC3 / TRPC6

TRP EXPRESSION SIGNATURE IN TUMOR-DERIVED ENDOTHELIAL CELLS: FUNCTIONAL ROLES IN PROSTATE CANCER ANGIOGENESIS

https://iris.unito.it/bitstream/2318/1801564/1/Poster%20PTEC_Montpellier%20e%20Bologna_sett%202019.pdf

TRP EXPRESSION SIGNATURE IN TUMOR-DERIVED ENDOTHELIAL CELLS: FUNCTIONAL ROLES IN PROSTATE CANCER ANGIOGENESIS

Abstract

Similar works

Full text

Available Versions

Institutional Research Information System University of Turin