Cagliero, Cecilia

Marengo, Arianna

Rubiolo, Patrizia

Sgorbini, Barbara

English

Institutional Research Information System University of Turin

15 October 2023AperTO - Archivio Istituzionale Open Access dell'Università di TorinoOriginal Citation:Derivatization-targeted analysis of amino compounds from Cardueae species by liquidchromatography tandem mass spectrometryTerms of use:Open Access(Article begins on next page)Anyone can freely access the full text of works made available as "Open Access". Works made availableunder a Creative Commons license can be used according to the terms and conditions of said license. Useof all other works requires consent of the right holder (author or publisher) if not exempted from copyrightprotection by the applicable law.Availability:This is the author's manuscriptThis version is available http://hdl.handle.net/2318/1842659 since 2022-02-21T17:24:46Z  Number (to be provided by Organizing Committee) Derivatization-targeted analysis of amino compounds from Cardueae species by liquid chromatography tandem mass spectrometry Arianna Marengo1,2, Larissa Silva Maciel2, Cecilia Cagliero1, Barbara Sgorbini1, Patrizia Rubiolo1, Koit Herodes2 1Department of Drug Science and Technology, University of Turin, Via P. Giuria , 10125; Turin, Italy; 2Institute of Chemistry, University of Tartu, Ravila 14a, 50411, Tartu, Estonia. Plants amino compounds (e.g. amino acids, biogenic amines) play important roles for the plant themselves and for human well-being. Reversed-phase liquid chromatography tandem mass spectrometry (RPLC-MS/MS) is one of the most used technique for amines analysis, but due to their structural characteristics, analyzing them in their native form remains challenging. Therefore, a prior derivatization step, to overcome this issue, is necessary [1,2]. In this work the use of neutral loss scan mode (NLS) is proposed for the first time in the investigation of amino compounds derivatized with diethyl ethoxymethylenemalonate (DEEMM) and analyzed by RPLC-MS/MS, to obtain an easy and straightforward method for the detection of known and unknown amines. The procedure has been firstly optimized on a model sample containing eight amino acids, with good results in terms of DEEMM derivatives detection and repeatability. The method was then successfully applied to the analysis of five Cardueae plant extracts, with 18 amino acids and 3 other amines being putatively identified. A statistical analysis on the collected data revealed a stability in the derivatives profiles among both the different selected species and samples from the same species with different geographical origins. The obtained results confirm the effective use of the proposed approach in the analysis of plants amino compounds for different purposes.  References [1] J.P. Violi, D.P. Bishop, M.P. Padula, J.R. Steele, K.J. Rodgers. TrAC - Trends Anal. Chem. 131 (2020) 116018. https://doi.org/10.1016/j.trac.2020.116018 [2] R. Dahl-Lassen, J. van Hecke, H. Jørgensen, C. Bukh, B. Andersen, J.K. Schjoerring, Plant Methods. 14 (2018) 1–9. https://doi.org/10.1186/s13007-018-0277-8 Conflict of Interest; Funding (Source, ID) The authors declare that they have no conflict of interest. This work was supported by the Estonian Research Council grant PUT1589, by the EU through the European Regional Development Fund (TK141 “Advanced materials and high-technology devices for energy recuperation systems”) and was carried out using the instrumentation at the Estonian Center of Analytical Chemistry (www.akki.ee). 

Derivatization-targeted analysis of amino compounds from Cardueae species by liquid chromatography tandem mass spectrometry

https://iris.unito.it/bitstream/2318/1842659/1/Marengo_GA2021.pdf

Derivatization-targeted analysis of amino compounds from Cardueae species by liquid chromatography tandem mass spectrometry

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