Abollino, O.

Giacomino, A.

Inaudi, P.

Malandrino, M.

English

Institutional Research Information System University of Turin

 Recent Developments in Pharmaceutical Analysis – RDPA2021 September 6th – 8th 2021, Modena, Italy  89  P22  Development of a Voltammetric Screening Method for Pharmaceutical Pollution in Waters   P. Inaudi1*, C. Mattalia1, M. Malandrino2, A. Giacomino1, O. Abollino1  1 Department of Drug Science and Technology, University of Torino, Via Pietro Giuria 9, 10125 Turin, Italy 2 Department of Chemistry, University of Torino, Via Pietro Giuria 5, Torino, 10125, Italy * Email: paolo.inaudi@unito.it  Pharmaceutical pollution is a growing concern all over the world. It is estimated that over 100,000 tonnes of active pharmaceutical ingredients (APIs) are consumed globally every year [1]. Medicinal products can enter the environment through manufacturing processes, consumption, improper disposal and lack of wastewater treatment plants designed to remove or degrade pharmaceuticals [2]. Although various APIs have been detected in all the environmental compartments, at concentrations in the range of sub-ng/l to more than the mg/l [2; 3], there is still insufficient knowledge about the ecotoxicological impact on exposed species [4; 5]. Moreover, the monitoring of pharmaceuticals in the environment is limited by the heterogeneity of these substances and their metabolites, their low concentrations, which could make quantification difficult, and the high analysis costs [2]. For these reasons, the availability of analytical methods that can provide fast and simple monitoring has become crucial. Hence, the aim of this research is to develop a low-cost and easy method that can be exploitable for on site analysis of waters. For this purpose, voltammetric methods were chosen; all the measurements were carried out with a portable potentiostat, using carbon paste electrodes (CPEs) as working electrode differential pulse voltammetry (DPV). This study focused on two of the most used analgesics and antipyretics, namely acetaminophen (APAP) and diclofenac (DCF), though the final purpose is to obtain a fingerprint of different APIs. Firstly, the responses of both APAP and DCF were tested at pH values ranging from 2 to 10, to observe the effects of pH on redox behaviour, sensitivity and repeatability. Furthermore, stability was explored through UV-VIS analysis and electro-degradation. Subsequent scans of DCF solutions lead to the detection of a second peak (pH range 5-10), plus a third one (pH range 2-4). The best analytical conditions, the use of the developed method to analyse pharmaceuticals trapped by polar organic chemical integrative samplers (POCIS) was investigated. HLB (Hydrophilic Lipophilic Balance) sorbent solid phase extraction (SPE) cartridges were used to reproduce POCIS on a small. Aqueous solutions of APAP and/or DCF were analysed before and after flowing through the SPE cartridge Next, the compounds retained by HLB were eluted with methanol and the eluate was collected in a Petri dish and mixed with graphite and paraffin oil, a carbon paste incorporating the compounds extracted from the HLB solid phase was obtained and a CPE was then prepared. Voltammograms reporting the redox signals of APAP and/or DCF were recorded. Being the analytes in solid phase, the method appears to gain in sensibility, but their quantification is not possible. The method is applicable as qualitative screening, to reveal the presence of pharmaceuticals trapped on passive samplers. The next steps of the research will be to study the electrochemical behaviour of other APIs and then apply the method to real water samples, to obtain a fingerprint revealing the presence, or the absence, of pharmaceuticals.   Recent Developments in Pharmaceutical Analysis – RDPA2021 September 6th – 8th 2021, Modena, Italy  90    The proposed method is fast, easy and cheap and enables to decrease the number of samples that need to be analysed with more sophisticated and expensive techniques.  References [1] KNAPPE project, Knowledge and Need Assessment on Pharmaceutical Products in Environmental Waters. Final report. 2008 [2] BIO Intelligence Service, Study on the environmental risks of medicinal products. Final Report prepared for Executive Agency for Health and Consumers. 2013 [3] Larsson, D.G.J. Phil Trans R Soc B 2014, 369, 20130571.  [4] OECD, Pharmaceutical Residues in Freshwater: Hazards and Policy Responses. OECD Studies on Water, OECD Publishing. 2019 [5] Crane, M.; Watts, C.; Boucard, T. Sci Total Environ 2006, 367, 23-41.   Dipartimento di Scienze della Vita - Università diModena e Reggio Emilia for GPP-SBIVia G. Campi 10341125 Modena (Italy)Dr. Paolo InaudiUniversity of TurinDepartment of Drug Science and Technologypaolo.inaudi@unito.it10100 TorinoItalyModena, Italy, 14/Sept/2021To Whom It May ConcernDear Madam or Sir,We confirm that Dr. Paolo Inaudi participated at Recent Developments in Pharmaceutical Analysis (RDPA2021), 6-8 September 2021, Modena - Italy.Dr. Paolo Inaudi is author/co-author of the following accepted contribution(s):P22 - Development of a Voltammetric Screening Method for Pharmaceutical Pollution in WatersAuthor(s): Inaudi, Paolo; Mattalia, Cecilia; Malandrino, Mery; Giacomino, Agnese; Abollino, OrnellaPresenting Author: Inaudi, PaoloStatus: PosterSession Details: Poster 1-3, Time: Monday, 06/Sept/2021: 5:30pm - 6:30pmWith best regards,Prof. Federica PellatiRDAPA2021 Conference ChairPowered by TCPDF (www.tcpdf.org)

Development of a Voltammetric Screening Method for Pharmaceutical Pollution in Waters

https://iris.unito.it/bitstream/2318/1885221/1/2021_RDPA_Inaudi_Poster.pdf

Development of a Voltammetric Screening Method for Pharmaceutical Pollution in Waters

Abstract

Similar works

Full text

Available Versions

Institutional Research Information System University of Turin