Benedicenti, Leontine

Bianchi, Ezio

Castro, Laura Espada

Cook, Audrey K.

De Marco, Viviani

Feldman, Edward C.

Fracassi, Federico

Gandini, Gualtiero

Golinelli, Stefania

Pöppl, Alan Gomes

Seo, Kyoung Won

Journal of Veterinary Internal Medicine

English

Lume 5.8

A B S T R A C TResearch Communicationsof the31st ECVIM-CA Online Congress1-4 September 2021The European College of Veterinary Internal Medicine – Companion Animals (ECVIM-CA) Congress and the Journal of Veterinary Internal Medicine(JVIM) are not responsible for the content or dosage recommendations in the abstracts. The abstracts are not peer reviewed before publication. The opin-ions expressed in the abstracts are those of the author(s) and may not represent the views or position of the ECVIM-CA. The authors are solely responsi-ble for the content of the abstracts.LIST OF ORAL RESEARCH COMMUNICATIONSESCG – European Society of Comparative GastroenterologyFriday 3 September14.25-14.40 ESCG-O-1 Bernard Comparative analysis of the distribution and severity of the lesions within the digestive tract infeline low-grade intestinal T-cell lymphoma or lymphoplasmacytic enteritis14.40-14.55 ESCG-O-2 Da Riz Short-term survival and associated factors after surgical intestinal biopsies in cats with chronicenteropathy14.55-15.10 ESCG-O-3 Wu Identification of bacteria in pancreas, liver, and bile of apparently healthy cats using nextgeneration 16S rRNA sequencing and standard bacteriological culture15.10-15.25 ESCG-O-4 Collier Investigating fecal microbial transplant in dogs with inflammatory bowel disease: A pilot study15.25-15.40 ESCG-O-5 Toresson Clinical effects of fecal microbiota transplantation in dogs with chronic enteropathy15.40-15.55 ESCG-O-6 Kuijlaars Faecal bile acid profiles in dogs with chronic enteropathies versus healthy controls15.55-16.10 ESCG-O-7 Sung Fecal fatty acid, cholesterol, and bile acid concentrations in cats with chronic enteropathy16.30-16.45 ESCG-O-8 Walker Metabolomic serum profiling in dogs with chronic enteropathy16.45-17.00 ESCG-O-9 Csukovich Taking the next step: Modelling infectious diseases in canine intestinal organoids17.00-17.15 ESCG-O-10 Thomson Retrospective analysis of the association between hepatic pathology and DGGR lipase in canineswith histologically normal pancreas17.15-17.30 ESCG-O-11 Méric Colorectal polypoid masses in dogs: Multicentre retrospective study of 53 cases17.30-17.45 ESCG-O-12 Dupont Suspected acute hemorrhagic diarrhea syndrome in out-patients: A preliminary study of diseaseseverity, treatment, outcome and client satisfaction17.45-18.00 ESCG-O-13 Tamura Low-dose oral cobalamin supplementation ameliorated in serum cobalamin concentrations indogs with chronic enteropathy when compared with small cell gastrointestinal lymphoma18.00-18.15 ESCG-O-14 Muradas Assessment of visceral pain in dogs with chronic enteropathy and its' effect on behaviour andowner-observed quality of lifeReceived: 13 October 2021 Accepted: 13 October 2021DOI: 10.1111/jvim.16289This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in anymedium, provided the original work is properly cited and is not used for commercial purposes.© 2021 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC. on behalf of the American College of Veterinary Internal Medicine.3080 J Vet Intern Med. 2021;35:3080–3204.wileyonlinelibrary.com/journal/jvimESVE-P-9 - European Society of Veterinary EndocrinologyEvaluation of the ACTH stimulation test using a low-dose of a depotformulation in healthy dogs and in dogs with naturally occurringCushing's syndromeL. Pérez-Lopez1, B. Blanco2, P. J. Ginel2, C. Melián31University Institute of Biomedical and Health Research, Las Palmas deGran Canaria, Spain; 2Animal Medicine and Surgery, University ofCordoba, Cordoba, Spain; 3Animal Pathology, University of Las Palmas deGran Canaria, Las Palmas de Gran Canaria, SpainThe adrenocorticotropic hormone (ACTH) stimulation test has beenwidely used to confirm the diagnosis of Cushing's syndrome (CS) andto monitor trilostane and mitotane treatment in dogs withCS. Previous studies have shown that the use of a depot formulationof tetracosactide may represent an alternative to the non-adsorbedsynthetic ACTH products in dogs. Nonetheless, its sensitivity to diag-nose CS requires further evaluation.The aims of this study were to propose reference intervals for cortisolvalues 1 hour after administration of a low-dose of depot ACTH inhealthy dogs and to evaluate the sensitivity of this test to detectCS. The sensitivity was also evaluated among different types of CSbased on an ultrasonographic classification.Forty-one healthy dogs (20 males, 21 females) with a median age of9 years were prospectively included. Additionally, 91 dogs with CS(31 males, 60 females) with a median age of 11 years were retrospec-tively included. Dogs with CS were ultrasonographically classified asfollows: 45 (49.4%) dogs with symmetrical adrenomegaly consistentwith pituitary-dependent hypercortisolism (PDH), 8 (8.8%) dogs withunilateral adrenomegaly and atrophy of the contralateral adrenal glandor unilateral or bilateral adrenomegaly with malignancy features con-sistent with adrenal-dependent hypercortisolism (ADH), 34 (37.4%)dogs with equivocal adrenal asymmetry (EAA) and 4 (4.4%) dogs withnormal adrenal thickness. A low-dose (5 μg/kg) of depot ACTH wasintramuscularly administered to all dogs and cortisol was measuredbefore and 1 hour post-ACTH.In healthy dogs, lower and upper limit of the 95% reference interval forpost-ACTH cortisol concentration and their confidence intervals, were4.4 (2.7-5.8) μg/dl and 18.4 (16.5-20.0) μg/dl, respectively. Post-ACTHcortisol concentration was above the reference interval in 81 out of91 dogs (89.0%) with CS. Forty two of the 45 (93.3%) dogs with PDH,5 of the 8 (62.5%) of dogs with ADH and 30 of the 34 (88.2%) of dogswith EAA had an elevated post-ACTH cortisol concentration consistentwith CS. Therefore, using a low-dose of a depot ACTH formulation, the1-hour post-ACTH cortisol concentration had good sensitivity to detectCS in dogs with PDH and EAA but low sensitivity to detect CS in dogswith ADH. In dogs with suspected CS and with ultrasound findings con-sistent with ADH, a normal ACTH stimulation test does not rule out CSand, in such cases, performing a different diagnostic test (ie, low-dosedexamethasone suppression test) is recommended.DisclosuresFinancial support for the ACTH stimulation tests performed in healthydogs was given by the BIO307 PAIDI Research Group, Junta deAndalucía.ESVE-P-10 - European Society of Veterinary EndocrinologyMyotonia associated with naturally occurring caninehypercortisolism: 30 cases (1984-2020)S. Golinelli1, F. Fracassi1, E. Bianchi2, A. Gomes Pöppl3,L. Benedicenti4, V. de Marco5, A. K. Cook6, L. Espada Castro7, K. WonSeo8, G. Gandini1, E. C. Feldman91Department of Veterinary Medical Sciences, University of Bologna,Ozzano dell'Emilia, Italy; 2Department of Veterinary Medical Sciences,University of Parma, Parma, Italy; 3Department of Animal Medicine,Federal University of Rio Grande do Sul, Rio Grande do Sul, Brazil; 4Department of Clinical Sciences and Advanced Medicine, University ofPennsylvania, Philadelphia, USA; 5Naya Especialidades, NayaEspecialidades, Sao Paulo, Brazil; 6Department of Small Animal ClinicalSciences, Texas A&M University, College Station, USA; 7School ofVeterinary Medicine, University of Glasgow, Glasgow, UK; 8Departmentof Veterinary Internal Medicine, Seoul National University, Seoul, South-Korea; 9Department of Medicine and Epidemiology, University ofCalifornia, Davis, USAMyotonia, a disorder characterized by delayed muscle relaxation aftervoluntary contraction or percussion, has been rarely associated withcanine hypercortisolism (HC). This retrospective multicenter studyaimed to describe the clinical findings and outcome of myotonia casesassociated with HC.Cases (n=30) of naturally occurring canine HC and myotonia wereretrospectively (1984-2020) included in the study. Twenty-twofemales (17 neutered) and 8 males (4 neutered) dogs were included.The most represented types were mixed breed (11/30), Poodle(7/30), and Dachshund (4/30). The median (range) body weight was7.75 (3.6-21) Kg. All dogs had pituitary-dependent HC. The mean(±standard deviation) age at the time of HC and myotonia diagnosiswas 10.7 years (±2.8) and 11.4 years (±2.8), respectively. In 10 dogs,HC was diagnosed first; in 19 cases, myotonia was diagnosed first,and in one dog, they were diagnosed simultaneously. Myotonic signs(stiffness, stilted gait, and hyperextension) developed first on thehindlimb in 18 dogs, first on the forelimbs in 5 dogs, and simulta-neously in all the limbs in 7 dogs. Pain was not reported in any caseby the referring veterinarian or the owner. Electromyography, per-formed in 11 dogs, showed the presence of myotonic discharges.Muscle/nerve biopsies, performed in 4 dogs, showed variation in mus-cle fiber size, moderate fiber fibrosis, and hypomyelination or demye-lination. Twenty-two dogs were treated with trilostane, 5 withmitotane, 2 with trilostane and then mitotane, and one withABSTRACT 3173 19391676, 2021, 6, Downloaded from https://onlinelibrary.wiley.com/doi/10.1111/jvim.16289 by CAPES, Wiley Online Library on [10/07/2023]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons Licensemelatonin. In 27/30 dogs HC signs improved after treatment. Myoto-nia treatment consisted of combined therapies including benzodiaze-pines (6/30), physiotherapy (4/30), cyclobenzaprine (3/30), mexiletine(2/30), dantrolene (2/30), nonsteroidal anti-inflammatory drugs(2/30), botulinum toxin (1/30), gabapentin (1/30), and methocarbamol(1/30). No dogs showed complete resolution of myotonic signs. How-ever, a mild improvement was noted in one dog treated with diaze-pam, one dog with mexiletine, and one dog with physiotherapy anddiazepam. Six dogs were lost to follow up, 10 dogs are still alive at thetime of writing, and 14 dogs died. Euthanasia was performed in 5/14dogs because of myotonic signs, in one because of HC signs, and in8 dogs the cause of death was unknown. The median survival timefrom the diagnosis of myotonia was 583 (125-4402) days.In conclusion, in most dogs, myotonic signs persisted despite the reso-lution of HC signs. However, our results suggest that the survival timeof dogs with HC-associated myotonia does not differ from that previ-ously reported for canine HC without myotonia.DisclosuresFederico Fracassi: Financial support: Dechra, MSD Speaking & consul-tancies: Â Boehringer Ingelheim, Dechra, MSD, Royal Canin, Hill's,Nestlé Purina, La Vallonea. Â Stefania Golinelli: Financial support: PhDscholarship funded by Dechra Speaking & consultancies: Â DechraAudrey K. Cook: Speaking & consultancies: Dechra, Zomedica.ESVE-P-11 - European Society of Veterinary EndocrinologyEvaluation of serum electrophoresis in dogs with pituitary-dependent hypercortisolismS. Golinelli, I. Buldrini, F. Dondi, F. FracassiDepartment of Veterinary Medical Sciences, University of Bologna,Ozzano dell'Emilia, ItalyChanges in serum electrophoresis (SE) of dogs with naturally occurringhypercortisolism (HC) have been rarely described. This retrospectivestudy aimed to compare SE of dogs with HC at diagnosis and healthydogs (HDs).Agarose gel electrophoresis of HDs and dogs with pituitary-dependent hypercortisolism (PDH), diagnosed at a referral veterinaryhospital, were retrospectively analyzed. PDH dogs and HDs weredefined based on clinical findings, CBC, serum biochemistry, urinalysis,and endocrine testing (PDH dogs only). SE fractions absolute values(AVs) were reported as mean± standard deviation (SD) or median(range) and compared between the two groups using unpaired T-Testor Mann-Whitney U Test, based on data distribution. Receiver operat-ing characteristic (ROC) curve analysis with the area under the ROCcurve (AUC) calculation was performed. P < 0.05 was consideredsignificant.Forty-nine PDH dogs and 34 HDs were included in the study. In PDHdogs the following SE fractions AVs resulted significantly lower com-pared to the HDs: albumin (3.18 ± 0.46 vs 3.42 ± 0.39 g/dL;P=0.0144), alfa-1 globulins (0.3 ± 0.07 vs. 0.33 ± 0.05 g/dL;P=0.0120), beta-2 globulins (0.66 ± 0.13 vs. 0.76 g/dL ± 0.18,P=0.0076) and gamma globulins (0.2 ± 0.12 vs. 0.62 ± 0.18 g/dL;P<0.0001). In PDH dogs alfa-2 globulins AVs were significantly higherin comparison to HDs (1.47 ± 0.26 vs. 0.92 ± 0.18 g/dL; P<0.0001).Most PDH dogs had the following SE fractions AVs within the labora-tory reference interval (RI): albumin (33/49), alfa-1 globulins, (41/49),beta-2 globulins (43/49). Most PDH dogs had gamma globulins belowthe RI (34/49) and all PDH dogs had alfa-2 globulins above the RI(49/49). The gamma:alfa-2 globulin (gamma:alfa-2) ratio was able todifferentiate PDH dogs from HDs. In particular, PDH dogs had signifi-cantly lower gamma:alfa-2 ratio in comparison to HDs (0.02 ± 0.01vs. 0.05 ± 0.01; P<0.0001). ROC curve analysis for gamma:alfa-2showed AUC of 0.97 and identified a cutoff of 0.025 as the best valueto discriminate between PDH dogs and HDs, with a sensitivity of 97%and a specificity of 86%.In conclusion, at SE, increased alfa-2 globulins, and decreased gamma-globulins and gamma:alfa-2ratio seemed to characterize dogs withPDH. The gamma:alfa-2 ratio showed promising results in differentiat-ing PDH dogs from HDs with a high sensitivity and good specificity.However, the absence of a control group of dogs with other diseases(i.e., inflammatory or neoplastic diseases) represents a limitation ofthe present study.DisclosuresFederico Fracassi: Financial support: Dechra, MSD Speaking & consul-tancies: Â Boehringer Ingelheim, Dechra, MSD, Royal Canin, Hill's,Nestlé Purina, La Vallonea. Â Stefania Golinelli: Financial support: Ph.D. scholarship funded by Dechra Speaking & consultancies: Â Dechra.ESVE-P-12 - European Society of Veterinary EndocrinologyTreatment and monitoring of naturally-occurring hypercortisolism byprimary care veterinarians: A Western European surveyM. F. Carvalho1, M. Bennaim2, S. Golinelli3, F. Fracassi3, C. Arenas4,M. Pérez-Alenza5, S. Galac6, C. T. Mooney7, R. O. Leal81Hospital Escolar Veterinário - Faculdade de Medicina Veterinária -Ulisboa, Lisboa, Portugal; 2Clinique Vétérinaire Aquivet, Bordeaux,France; 3Department of Veterinary Medical Science, University ofBologna, Bologna, Italy; 4Anicura Hosp. Vet. Valencia Sur, Valencia,Spain; 5Hosp. Clinico Vet., Complutense University, Madrid, Spain; 6Department of Clinical Sciences, Faculty of Veterinary Medicine, UtrechtUniversity, Utrecht, Netherlands; 7Small Animal Clinical Studies, School ofVeterinary Medicine, University College Dublin, Dublin, Ireland; 8CIISA -3174 ABSTRACT 19391676, 2021, 6, Downloaded from https://onlinelibrary.wiley.com/doi/10.1111/jvim.16289 by CAPES, Wiley Online Library on [10/07/2023]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License

Myotonia associated with naturally occurring canine hypercortisolism : 30 cases (1984-2020)

https://lume.ufrgs.br/bitstream/10183/263676/1/001173635.pdf

Myotonia associated with naturally occurring canine hypercortisolism : 30 cases (1984-2020)

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