Monoclonal antibody (Mab) MOvi 8 preferentially reacts with gynecolo @ carcinomas.We haveanalyzedthe character istics of munne MOvi 8 (m-MOvl 8) and chimeric MOvi 8 (c MOvi 8). We found no differences in affinity and binding to IGROV1cellsbetweenc-MOvl 8 a

Carla F. Molthoff

Epic Boven

Herbert M. Pinedo

Marrije R. Buist

Peter Kenemans

Monoclonal antibody (Mab) MOv18 preferentially reacts with gynecological carcinomas. We have analyzed the characteristics of murine MOv18 (m-MOv18) and chimeric MOv18 (c-MOv18). We found no differences in affinity and binding to IGROV1 cells between c-MOv18 as IgG and F(ab')2 fragments and m-MOv18. In nude mice bearing IGROV1 xenografts, maximum tumor uptake 6-15 hr after i.v. injection of radiolabeled m-MOv18 IgG, c-MOv18 IgG, c-MOv18 F(ab')2 and a control IgG, 2C7, was 10%, 11%, 3% and 4.5% of the injected dose/g (%ID/g), respectively. M- and c-MOv18 IgG retained this level for several days, while c-MOv18 F(ab')2 and 2C7 cleared rapidly from the tumor. Uptake in normal tissues was low, with the exception of high uptake in kidneys for c-MOv18 F(ab')2. Tumor/blood ratios for c-MOv18 F(ab')2 were sixfold higher than for IgG. Radiation absorbed doses to tumor tissue delivered by 10 microCi iodinated m-MOv18 IgG, c-MOv18 IgG and c-MOv18 F(ab')2 were 39 cGy, 49 cGy and 5 cGy, respectively. A cocktail of 125I-c-MOv18 IgG and 131I-c-MOv18 F(ab')2 injected i.v. into an ovarian cancer patient, localized specifically in the tumor. Ovarian cancer tissue samples obtained 2 days postinjection showed a mean uptake of 1.2 x 10(-3) and 2.7 x 10(-3) %ID/g for c-MOv18 IgG and c-MOv18 F(ab')2, respectively. Results from these in vitro and in vivo experiments indicate that c-MOv18 has promise as a Mab for clinical us

Molthoff, C. F.

Buist, M. R.

Kenemans, P.

Pinedo, H. M.

Boven, E.

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Experimental and clinical analysis of the characteristics of a chimeric monoclonal antibody, MOv18, reactive with an ovarian cancer-associated antigen

Experimental and Clinical Analysis of the Characteristics of a Chimeric Monoclonal Antibody, M0v18, Reactive with an Ovarian Cancer-AssociatedAntigen

Experimental and Clinical Analysis of the Characteristics of a Chimeric Monoclonal Antibody, M0v18, Reactive with an Ovarian Cancer-AssociatedAntigen

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