This study aimed to address current gaps in the literature on the impacts of Mental Health First Aid (MHFA) training by comparing long-term outcomes on diferent cohorts. University students studying health and non-health degrees, university staf and community members who had completed MHFA within the past three years completed a survey to assess their mental health literacy, and their use of knowledge and skills acquired through the training. The study found few signifcant diferences between the diferent group outcomes and between diferent time points post-completion of MHFA. This study found that although participant-reported measures show positive outcomes from MHFA, further research on the common tools used to rate mental health literacy and how mental health literacy impacts behaviours and help-seeking would provide greater insight into the outcomes of running MHFA as a mental health prevention and early intervention program at universities