A primary mode of human social behavior is face-to-face interaction. In this study, we investigated the characteristics of gaze and its relation to speech behavior during video-mediated face-to-face interactions between parents and their preadolescent children. 81 parent–child dyads engaged in conversations about cooperative and conflictive family topics. We used a dual-eye tracking setup that is capable of concurrently recording eye movements, frontal video, and audio from two conversational partners. Our results show that children spoke more in the cooperation-scenario whereas parents spoke more in the conflict-scenario. Parents gazed slightly more at the eyes of their children in the conflict-scenario compared to the cooperation-scenario. Both parents and children looked more at the other's mouth region while listening compared to while speaking. Results are discussed in terms of the role that parents and children take during cooperative and conflictive interactions and how gaze behavior may support and coordinate such interactions