Additional file 1 of Chemoautotrophic production of gaseous hydrocarbons, bioplastics and osmolytes by a novel Halomonas species

Abstract

Additional file 1: Figure S1. Plasmid map for propane production in Halomonas. Figure S2. Tolerance of Halomonas isolates for salinity, pH and butyric acid. Figure S3. Growth of H. rowanensis in mineral-based media using polluted water with and without exogenous carbon sources. Figure S4. Superimposition of AlphaFold predicted structures of reverse TCA cycle proteins and the closest DALI homology match. Figure S5. Sulfur oxidation systems for energy generation. Figure S6. Ectoine production by Halomonas rowanensis in the thiosulfate minimal medium with a range of salinity. Table S1. Partial 16S rDNA sequence analysis of ‘Old Biot’ brine spring isolates. Table S2. Extended PHB assay data for Figs. 2C and . Table S3. Putative carbon fixation cycle genes identified within the genome of H. rowanensis by protein sequence homology and AlphaFold structural homology. Table S4. Putative sulfur metabolism genes identified within the genome of H. rowanensis by protein sequence homology and AlphaFold structural homology