Acaricides have played a pivotal role in the control of the tick Rhipicephalus (Boophilus) microplus. As a consequence of extensive use, R. (B.) microplus has developed resistance to all major classes of acaricides, especially to pyrethroids. The thesis describes field and laboratory research undertaken in Yucatan, Mexico and Liverpool, UK to investigate pyrethroid resistance (PR) in R. (B.) microplus. For the first time gDNA of R. (B.) microplus was sequenced around the target single nucleotide polymorphism (SNP) in the sodium channel gene associated with PR and three new SNPs were identified