Expression of MMP-2, -8 and -9 in Inflamed Pulp

Abstract

2010 IADR/PER General Session (Barcelona, Spain)Objective: to assess the presence of MMP-2, MMP-8 and MMP-9 in different stags of pulp inflammation. Null hypothesis: 1- the expression pattern of MMP-2, MMP-9 and MMP-8 does not change in respect to the stages of pulp inflammation; 2- there is no difference among MMP-2, MMP-9 and MMP-8 expression, considering the same period of pulp inflammation. Methods: Specimens were divided in groups according to criteria adopted of subjective and objective clinical diagnosis of pulp condition: GI- Control = healthy pulp (n=7); GII- Reversible Pulpitis (n=4); GIII– Pulpitis in transition stage (n=4); GIV- Irreversible Pulpitis/Necrosis (n=8). After extraction, teeth were cut below the cement-enamel junction, fixed with 10% formalin for 48h and washed in water (24h). Each tooth was 4µm thickness sectioned, adhered to silane coated glass slides and immunostained (IS). The following scores were used for MMPs IS semi-quantitative analysis: 0= absent; 1= mild; 2= moderate; 3= intense. Kruskal-Wallis nonparametric statistical test (pGIV (pGIV (pGI (pGIV (pGIV (pGI (pGII (pGI (pGI (pGII (pGII (pMMP9 (pMMP-9 (pMMP-2 (pMMP-2 (p<0.01), MMP-2=MMP-8 and MMP-9=MMP-8. Conclusions: The expression of MMP-2, MMP-9 and MMP-8 varied according to the stage of pulp inflamation, in bolth OL and DP. MMP-2 is heigher expressed in healthy pulp. The heighest MMP-9 expression is related to inflamed pulp. MMP-8 is present in bolth health and inflamed pulp