Provided genotoxicity data for the Irtysh river valey (West Siberia). To assess genotoxic effects used Allium test. Ana-telophase chromosomal aberration assay and micronuclei test we performed to determine genotoxic effects. Obtained data for mitotic index and other phase indexes. The data allow other researchers to conduct a comprehensive analysis of the genotoxicity of natural waters on the landscape gradient of a river valley (terrace-floodplain-river) and an analysis of the possible causes of the observed effects.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV