Data from: Cuticular hydrocarbon divergence in the jewel wasp Nasonia: Evolutionary shifts in chemical communication channels?

Abstract

The data package contains eight datasets: - CSV files containing information on the GC-MS data (GC: 7890A; MS: 5975C; Agilent Technologies, Waldbronn, Germany) for all Nasonia samples, obtained with “Enhanced Chemstation”, G1701AA, Version A.03.00. Columns are identified as follows: Peak number, average retention time, peak type, peak width, peak area (integrated absolute value), start retention time, end retention time. Further description of the data generation see Materials and Methods section in the paper. - CSV files containing information on the GC-MS data (GC: 7890A; MS: 5975C; Agilent Technologies, Waldbronn, Germany) for the Trichomalopsis outgroup samples, obtained with “Enhanced Chemstation”, G1701AA, Version A.03.00. Columns are identified as follows: Peak number, average retention time, peak type, peak width, peak area (integrated absolute value), start retention time, end retention time. Further description of the data generation see Materials and Methods section in the paper. - CHC peak areas standardized with the normalization method of the function "decostand" of the community ecology R package “vegan” (Dixon, 2003), for a more detailed description see Materials and Methods section in the paper. - Functions of the linear discriminant analysis (DA) calculated from the normalized CHC data on the Nasonia samples, first three functions plotted simultaneously in Figure 1 in the paper, further details on the DA see Materials and Methods and Results, respectively. - Phylogenetic distance matrix, used in comparison with the average manhattan chemical distances between the Nasonia species based on the relative differences between their normalized CHC peak areas, performed separately for males and females (Fig. 2 and 3, respectively). The molecular distances are based on the Nasonia phylogeny, which are themselves based on the genetic divergence of a mitochondrial gene, COI sequences retrieved from NCBI, pairwise p-distances calculated with MEGA 3.0 (Werren et al., 2010) For further information, please see Materials and Methods section in the paper as well as the Tables S2 and S3, which give the average manhattan distances the phylogenetic distances were compared to. - Single results of the Mantel tests, performed five times for both male CHC divergence vs. Nasonia phylogeny and female CHC divergence versus Nasonia phylogeny, all five p values and their respective averages are given for both combinations, as well as the r value. Each single test was performed with 9999 permutations. - Raw data on the behavioral assays, focussing on N. oneida and N. giraulti males (Fig. 4). Whether a copulation has occurred or not is given in the second column for each pairing in a binary coding (1 = yes, 0 = no), and the average number of occurred copulations is given for each pairing with untreated and treated female dummies. Species affiliation and treatment of female dummies indicated in the caption of each pairing. Male species affiliation can be found in the main caption on each spread sheet. Furthermore, the male arrestment time has been recorded (third column), but those data have not been used in this study. Mating results on two further N. giraulti strains are given as well. - Relative (percentages) distribution of CHC compound classes among the species and sexes of Nasonia and the outgroup Trichomalopsis, data underlying Fig. S2