One of the main limitations of the cucurbit crops production is the powdery mildew disease,
caused by the biotrophic fungus Podosphaera xanthii. An integrated management, using
several strategies, is carried to control the disease but the application of fungicides is the most
effective one. The problem is that P. xanthii has been classified by the Fungicide Resistance
Action Committee (FRAC) as a pathogen with a high risk of resistance developing, in addition
of the strong restrictions on the use of phytosanitary products at a European level. For this
reason, new phytosanitary tools are necessary to allow a sustainable control of this
devastating disease such as the use of the RNA interference (RNAi) technology. In this work,
dsRNA targeting a P. xanthii gene, which encodes a protein that appears to be essential for
the correct assembly of the fungal cell wall, was evaluated. Preliminary gene silencing results
have shown a significant reduction of fungal development on melon plants suggesting that this
gene may be a promising target for the control of powdery mildew of cucurbits.
This work has been funded by AEI (PID2019-107464RB-C21).Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech
