'Universidad Nacional Hermilio Valdizan - Facultad de Ciencias de la Educacion'
Abstract
Introduction. A workable strategy is bioaugmentation, which involves introducing certain bacteria in sufficient quantities to promote biodegradation. This study focuses on isolating and utilizing malodor-reducing bacteria from fecal wastes obtained from a poultry farm in Ashi, Ibadan. Methods. Standard methods were employed to isolate and identify species of Pseudomonas and Bacillus. Quantitative detection of hydrogen sulfide gas and other relevant parameters was performed using MSA Orion and Multi Gas Detector. Hydrogen sulfide (H2S) release was quantitatively monitored during fermentation, considering varying loads of inocula. Results. The bacterial isolates comprised Pseudomonas aeruginosa, P. fluorescens, P. putida, Bacillus fastidiosus, B. licheniformis, B. megaterium, B. subtilis, B. sphaericus, and B. thuringiensis. Odor levels varied based on inocula load and fermentation duration. In batches with Pseudomonas, hydrogen sulfide was undetectable after two days, while Bacillus-inoculated batches required ten days. The formation of microbial mats and subsequent decrease in H2S content contributed to malodor reduction. Notably, fluorescent pseudomonas exhibited successful mineralization during the treatment of fecal waste. Conclusion. Pseudomonas isolates demonstrated superior effectiveness in odor reduction compared to Bacillus isolates
