V.cholerae

Abstract

This was imaged to study Yi-wei Msh in V.cholerae. This strain expresses Msh pilus but not any other pili.<strong>Tilt Series Date:</strong> 2017-01-06</p> <strong>Data Taken By:</strong> Poorna Subramanian</p> <strong>Species / Specimen:</strong> Vibrio cholerae</p> <strong>Strain:</strong> O1 biovar El Tor</p> <strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 160.0 eV/Ų, defocus: -6.0 μm, magnification: 27500x. </p> <strong>Microscope:</strong> Caltech Polara</p> <strong>Acquisition Software:</strong> UCSFTomo</p> <strong>Upload Method:</strong> pipeline</p> <strong>Processing Software Used:</strong> Raptor</p> <strong>Collaborators and Roles:</strong> Yi-wei</p> <strong>Purification / Growth Conditions / Treatment:</strong> growth and freezing conditions: pg 95-97 in notebook. next time for vitrobot use: 6, 2.5, 1. 3.5 and 4 ul sample volume</p> <strong>Sample Preparation:</strong> single tilt. constant angular increment, step: 1.0. tilt range: -60, 60. dosage: 160e/A2. defocus: -6um. magnification: 27500.</p> psa2017-01-06-47_slicer17369.jpg: secreted vesicles?Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-01-06-47</p>atlas50025_full.rec, Reconstruction (Pixel Size 1.56 nm), 712.7 MB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-01-06-47/3dimage_80166/atlas50025_full.rec" > <i class="download icon"></i> Download </a></p> atlas50025.mrc, Tilt Series (Pixel Size 0.39 nm), 3.4 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/psa2017-01-06-47/rawdata/atlas50025.mrc" > <i class="download icon"></i> Download </a></p&gt