The non-linear signal generation in Magnetic Particle Imaging (MPI) using magnetic nanoparticles as tracer materials is still not fully explained and a far-reaching research area. Magnetic Particle Spectroscopy (MPS) was developed to investigate the particle behavior in high externally applied magnetic field strengths and to derive mathematical models which describe the physical processes in MPI in detail. A new MPS setup was built which allows measurements between -13 °C and +114 °C in order to investigate the temperature dependence of the harmonics spectra. Temperature-dependent MPS measurements of diluted FeraSpinTM XL, either as suspension or freeze-dried in a mannitol matrix, using the new setup are shown and exemplarily discussed
