Background: During the detorsion of a torsioned ovary, oxidant agents
are released and melatonin as an antioxidant can reduce ischemia. We
studied the histopathological changes after using melatonin on
experimental torsioned ovary in cat. Objective: The aim of this
experimental study was to investigate the effects of Melatonin on
histopathological changes in torsion – detorsion injury in cat
ovary. Materials and Methods: An adnexal torsion – detorsion
model was created by using 20 adult cats randomly divided equally in to
2 groups of Saline and Melatonin. Ischemia was induced by iathrogenic
360° clockwise torsion of the cat adnex for 3 hr. Reperfusion was
achieved for 3 hr. Melatonin or saline were injected intra peritoneally
(10mg/kg) 30 min before ovarian detorsion in both groups. After 3 hr of
ovarian detorsion, ovarian tissue was removed and fixed in 10% formalin
solution, embedded in paraffin and evaluated for ischemic indices.
Results: Histological examination showed a significant improvement in
ovarian morphology in the melatonin treated cats. Edema and
vasoconstriction in saline group were more severe than Melatonin group
(p-value = 0.009). Hemorrhage and leukocyte infiltration were also more
obvious in saline group (p-value 0.0018) Conclusion: Our results
demonstrated that Melatonin administration reduced ovarian
histopathological damage due to oxidative injury associated with
torsion
